Related Experiment Video

Updated: May 12, 2026

Culture Methods to Determine the Limit of Detection and Survival in Transport Media of Campylobacter Jejuni in Human Fecal Specimens
08:23

Culture Methods to Determine the Limit of Detection and Survival in Transport Media of Campylobacter Jejuni in Human Fecal Specimens

Published on: March 10, 2020

First report of ciprofloxacin-resistant Salmonella Enteritidis isolated from human cases in Morocco

Hajar Elmourabit1, Bouchra Karraouane2, Brahim Bouchrif2

  • 1Laboratory of Plant Biotechnology, Faculty of Sciences, Ibn Zohr University, box 8106, Agadir, Morocco.

Journal of Infection in Developing Countries
|May 11, 2026
PubMed
Abstract

Insights

The first ciprofloxacin-resistant Salmonella Enteritidis strain was identified in Morocco. This finding underscores the need for enhanced surveillance of antimicrobial resistance in Salmonella to prevent further spread.

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Genomics

Background:

  • Salmonella Enteritidis is a major cause of global foodborne and invasive infections.
  • Ciprofloxacin is a key treatment for invasive Salmonella, but resistance is a growing concern.
  • No ciprofloxacin-resistant S. Enteritidis had been reported in Morocco prior to this study.

Purpose of the Study:

  • To report the first instance of ciprofloxacin-resistant Salmonella Enteritidis in Morocco.
  • To characterize the genetic basis of ciprofloxacin resistance in this isolate.
  • To emphasize the need for improved surveillance of antimicrobial resistance.

Main Methods:

  • Sixty Salmonella strains were isolated from clinical samples in Marrakech (2018-2024).
  • Antimicrobial susceptibility testing (disk diffusion) and whole-genome sequencing were performed.
  • Genomic analysis identified resistance and virulence genes using ResFinder, PlasmidFinder, and VFDB.

Main Results:

  • Three isolates (5%), including strain S54, exhibited resistance to ciprofloxacin.
  • Genomic analysis revealed a gyrA D87Y mutation and efflux genes (mdsA, mdsB).
  • Virulence genes located in SPI-1 and SPI-2 were also detected.

Conclusions:

  • This study documents the first ciprofloxacin-resistant S. Enteritidis strain identified in Morocco.
  • The findings highlight the critical need for enhanced molecular surveillance.
  • Preventive strategies against fluoroquinolone-resistant Salmonella are urgently required.
Keywords:
S. Enteritidisciprofloxacinresistancewhole-genome sequencing

More Related Videos

Loop-Mediated Isothermal Amplification for Screening Salmonella in Animal Food and Confirming Salmonella from Culture Isolation08:24

Loop-Mediated Isothermal Amplification for Screening Salmonella in Animal Food and Confirming Salmonella from Culture Isolation

Published on: May 20, 2020

Related Concept Videos

Reservoir of Infection01:30

Reservoir of Infection

Infectious diseases arise from intricate interactions between pathogens and their reservoirs. A reservoir of infection refers to the natural habitat where a pathogen lives, grows, and multiplies, serving as a continual source of infection. Reservoirs are broadly classified as either living or nonliving, and each plays a unique role in disease transmission, significantly influencing public health interventions and control strategies.Humans act as reservoirs for a wide array of pathogens,...
Investigation of Disease Outbreaks01:23

Investigation of Disease Outbreaks

Multistate foodborne outbreaks pose significant public health risks and require meticulous investigation to identify sources and implement control measures. The Centers for Disease Control and Prevention (CDC) utilizes a dynamic seven-step process for these investigations, integrating data from laboratories, interviews, and environmental assessments to protect public health.Outbreak Detection: The detection of multistate outbreaks typically begins with PulseNet, the CDC's national laboratory...