Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 12, 2026

Intracellular Staining and Flow Cytometry to Identify Lymphocyte Subsets within Murine Aorta, Kidney and Lymph Nodes in a Model of Hypertension
09:20

Intracellular Staining and Flow Cytometry to Identify Lymphocyte Subsets within Murine Aorta, Kidney and Lymph Nodes in a Model of Hypertension

Published on: January 28, 2017

Identification of ATF3 as a Potential Key Biomarker for Hypertensive Nephropathy via Single-Cell Transcriptome

Fangyan Tan1, Rong Hu1, Jianlin Du1

  • 1Department of Cardiology, The Second Affiliated Hospital of Chongqing Medical University, 74 Linjiang Road, Yuzhong, Chongqing, 400010, China.

Inflammation
|May 11, 2026
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Synergistic impact of nondipper heart rate and blood pressure on left ventricular hypertrophy in essential hypertension.

Clinical and experimental hypertension (New York, N.Y. : 1993)·2026
Same author

Impact of maternal lipopolysaccharide and polyinosinic-polycytidylic acid-induced infections on offspring cardiac development: Mitochondrial dysfunction and metabolic alterations.

Genes & diseases·2026
Same author

Interleukin-1/Toll-like receptor signaling potentiates macrophage olfactory receptor 2-driven atherosclerosis.

Cell reports·2025
Same author

Single-Cell Transcriptomics Unravels Growth Factor Erv1-Like Mediated Ferroptosis as a Key Driver of Intestinal Epithelial Dysfunction in Ulcerative Colitis.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2025
Same author

Effect of Sacubitril-Valsartan on Transcriptomic Changes in Lung Tissue of Spontaneously Hypertensive Rats: A Multi-omics Study Based on RNA-Seq Transcriptome Analysis.

American journal of hypertension·2025
Same author

Augmenter of liver regeneration (ALR) can regulate iron homeostasis through the INOS/NO/SLC39A14 pathway to reduce ferroptosis in acute kidney injury.

The FEBS journal·2025

Hypertensive Nephropathy (HTN) damages kidneys, leading to end-stage renal disease (ESRD). This study identifies activating transcription factor 3 (ATF3) as a key biomarker and therapeutic target for HTN, crucial for early detection and treatment.

Area of Science:

  • Nephrology
  • Molecular Biology
  • Genomics

Background:

  • Hypertensive Nephropathy (HTN) is a major cause of end-stage renal disease (ESRD).
  • Current diagnostic methods lack specific biomarkers for early renal injury detection and molecular characterization.
  • The complex pathogenesis of HTN involves diverse cellular alterations.

Purpose of the Study:

  • To identify novel biomarkers for early detection of Hypertensive Nephropathy.
  • To elucidate the molecular mechanisms underlying renal injury in HTN.
  • To explore potential therapeutic targets for HTN.

Main Methods:

  • Integrated analysis of single-cell RNA sequencing (scRNA-seq) data from HTN patients and controls.
  • Cell subpopulation identification, differential gene expression analysis, and functional enrichment.
Keywords:
Activating transcription factor 3Hypertensive nephropathyRenal tubular epithelial cellsSingle-cell RNA sequencing

More Related Videos

Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
11:11

Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting

Published on: March 17, 2023

Related Experiment Videos

Last Updated: May 12, 2026

Intracellular Staining and Flow Cytometry to Identify Lymphocyte Subsets within Murine Aorta, Kidney and Lymph Nodes in a Model of Hypertension
09:20

Intracellular Staining and Flow Cytometry to Identify Lymphocyte Subsets within Murine Aorta, Kidney and Lymph Nodes in a Model of Hypertension

Published on: January 28, 2017

Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
11:11

Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting

Published on: March 17, 2023

  • Non-negative matrix factorization (NMF), pseudotime trajectory, and transcription factor regulatory network analysis.
  • In vivo and in vitro experimental validation.

    • Main Results:

    • A regulatory network centered on activating transcription factor 3 (ATF3) was identified in renal tubular epithelial cells of HTN tissues.
    • ATF3 was significantly upregulated in HTN, functioning as a critical stress response factor.
    • ATF3 regulates oxidative stress signaling pathways via FOSB, contributing to HTN pathogenesis.

    Conclusions:

    • ATF3 is a promising key biomarker for Hypertensive Nephropathy, enabling early diagnosis.
    • The study provides a novel molecular target, ATF3, for the diagnosis and targeted therapy of HTN.
    • Understanding ATF3's role in oxidative stress offers new avenues for HTN treatment.