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Closed Chest Biventricular Pressure-Volume Loop Recordings with Admittance Catheters in a Porcine Model

Closed Chest Biventricular Pressure-Volume Loop Recordings with Admittance Catheters in a Porcine Model

Published on: May 18, 2021

Fixed Volume-Intercept Approach for Serial Contractility Assessment with Closed-Chest Pressure-Volume Loops in a

Oskar Kjærgaard Hørsdal¹, Kristoffer Berg-Hansen², Nigopan Gopalasingam³

¹Department of Cardiology, Aarhus University Hospital; Department of Clinical Medicine, Aarhus University; osho@clin.au.dk.

Journal of Visualized Experiments : Jove

|May 11, 2026

Summary

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This study introduces a new method to assess left-ventricular (LV) contractility using a fixed end-systolic volume intercept (V₀) for end-systolic elastance (Ees) estimation. This approach avoids risky preload reduction in unstable animal models, enabling reliable contractility tracking.

Area of Science:

Cardiovascular Physiology
Hemodynamics
Animal Models

Background:

The end-systolic pressure-volume relationship (ESPVR) is a key indicator of left-ventricular (LV) contractility.
Traditional ESPVR assessment via inferior vena cava (IVC) occlusions can cause instability in critical conditions like cardiogenic shock.
Novel methods are needed for safe and reliable inotropic state assessment in fragile hemodynamic states.

Purpose of the Study:

To present a practical protocol for estimating LV contractility in large animals.
To enable serial assessment of contractility without repeated, destabilizing preload manipulations.
To provide a method suitable for unstable animal models where traditional techniques are contraindicated.

Main Methods:

A novel approach fixing the volume-axis intercept (V₀) of the ESPVR.

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An Isolated Working Heart System for Large Animal Models

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Last Updated: May 13, 2026

Closed Chest Biventricular Pressure-Volume Loop Recordings with Admittance Catheters in a Porcine Model

Closed Chest Biventricular Pressure-Volume Loop Recordings with Admittance Catheters in a Porcine Model

Published on: May 18, 2021

An Isolated Working Heart System for Large Animal Models

An Isolated Working Heart System for Large Animal Models

Published on: June 11, 2014

Single-beat estimation of end-systolic elastance (Ees) from LV pressure-volume loops.

Application in a porcine model of cardiogenic shock under acute, closed-chest conditions.

Main Results:

The fixed-V₀ method reliably tracked load-independent changes in myocardial performance.
The protocol successfully avoided arrhythmias and hemodynamic instability induced by preload reduction.
This method proved feasible and physiologically sound for serial contractility assessment in unstable models.

Conclusions:

The fixed-V₀ method offers a practical and safe alternative for assessing LV contractility in acute, unstable large-animal studies.
This technique balances methodological rigor with experimental safety, particularly when preload manipulation is unsafe.
The assumption of a fixed V₀ is a simplification and requires re-estimation if significant ventricular remodeling or pressure changes occur.