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Related Concept Videos

Pulmonary Tuberculosis II01:28

Pulmonary Tuberculosis II

Tuberculosis, or TB, is a bacterial infectious disease caused by Mycobacterium tuberculosis. While its primary impact is on the lungs, leading to pulmonary tuberculosis, it can also affect various other organs, a condition referred to as extrapulmonary tuberculosis.
Here is a detailed explanation of its pathophysiology:
Transmission: The process begins when a person inhales droplet nuclei containing M. tuberculosis. These are typically released into the air when an individual with pulmonary or...
Pulmonary Tuberculosis I01:29

Pulmonary Tuberculosis I

Tuberculosis, often called TB, is a contagious illness primarily caused by Mycobacterium tuberculosis. It mainly affects the lung parenchyma but can also impact other body parts.
Causative Organism
The primary infectious agent causing tuberculosis is Mycobacterium tuberculosis, a slow-growing, acid-fast, aerobic rod that exhibits sensitivity to heat and ultraviolet light. Instances of Mycobacterium bovis and Mycobacterium avium contributing to the development of TB infection are rare.
Mode of...
Pulmonary Tuberculosis III01:31

Pulmonary Tuberculosis III

Tuberculosis (TB) is a contagious infection primarily affecting the lung parenchyma but which can also affect other body parts. TB can be classified based on disease development, presentation, and the affected anatomical site.
The first classification is based on the development of the disease, and it includes the following categories:
Defense Against Bacterial Pathogens01:31

Defense Against Bacterial Pathogens

The human immune system is a complex network of cells, tissues, and organs that work together to defend the body against bacterial infections. It consists of various immune cells, each playing a specific role in the defense mechanism.
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
Pulmonary Tuberculosis IV01:26

Pulmonary Tuberculosis IV

Tuberculosis, more commonly referred to as TB, is an infectious disease stemming from Mycobacterium tuberculosis. While it primarily impacts the lungs, TB can also affect other body areas. Given its severity and global impact, timely and accurate diagnosis is crucial for controlling its spread and improving patient outcomes.
Several diagnostic approaches are used to detect TB. The conventional method is the Tuberculin Skin Test (TST), also known as the Mantoux test. However, this method has...
Pulmonary Tuberculosis V01:28

Pulmonary Tuberculosis V

Medical management of tuberculosis (TB) patients involves a comprehensive approach that includes diagnosis, treatment, and monitoring. The specific strategies can vary depending on the type of tuberculosis (latent or active), the patient's overall health status, and other considerations.
Latent tuberculosis infection occurs when TB bacteria are present in a person's body, but are not causing illness or symptoms. It is not contagious, and preventive treatment is crucial to avoid the progression...

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Fluorescence Assays for the Study of Mycobacterium tuberculosis Interaction with the Immune Receptor SLAMF1
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Platelet-leucocyte interactions drive MMP-mediated tissue damage in tuberculosis.

Daniela E Kirwan1, Deborah L W Chong1, Oscar Gayoso2

  • 1Institute for Infection & Immunity, City St. George's, University of London, London, United Kingdom.

Plos Pathogens
|May 11, 2026
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Platelets drive inflammation and tissue damage in tuberculosis by interacting with monocytes via P-selectin and PSGL-1, increasing matrix metalloproteinase (MMP) secretion. Targeting this platelet-leukocyte pathway offers a potential host-directed therapy for tuberculosis.

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Published on: September 18, 2020

Area of Science:

  • Immunology
  • Pulmonology
  • Pathology

Background:

  • Tuberculosis (TB) involves inflammation and matrix metalloproteinase (MMP) activity, leading to tissue damage.
  • Platelets are increasingly recognized as key inflammatory drivers.
  • Platelet-leukocyte aggregates, particularly involving monocyte PSGL-1 and platelet P-selectin, may mediate TB-related tissue destruction.

Purpose of the Study:

  • To investigate the role of platelet-leukocyte interactions in tuberculosis pathogenesis.
  • To assess the impact of these interactions on MMP secretion and gene expression.
  • To explore potential host-directed therapeutic targets in TB.

Main Methods:

  • Utilized a platelet-monocyte co-culture model to study interactions.
  • Examined M.tb-infected and control lymph node tissue via immunofluorescence microscopy.
  • Quantified platelet aggregation, platelet-monocyte aggregates (PMA), platelet-neutrophil aggregates (PNA), and receptor expression in TB patients, healthy controls, and respiratory symptomatic patients using flow cytometry and light transmission aggregometry.

Main Results:

  • M.tb-infected monocytes co-cultured with platelets showed significantly increased MMP-1 and MMP-10 secretion and mmp1 gene expression.
  • MMP-1 secretion was enhanced by platelet-derived soluble factors and monocyte PSGL-1 ligation.
  • Abundant platelets were found in M.tb-infected lymph nodes, localizing to monocytic PSGL-1, unlike in controls.
  • Ex vivo platelet aggregation was reduced in TB patients compared to healthy controls.
  • PMA levels were elevated in TB and respiratory symptomatic patients, while PNA levels were higher only in TB patients.
  • Platelet P-selectin expression, PMA, and PNA correlated, independent of GPIIb/IIIa, suggesting a non-thrombotic pathway.

Conclusions:

  • PSGL-1/P-selectin mediated platelet-leukocyte interactions are crucial drivers of inflammation and MMP secretion in pulmonary TB.
  • Platelets are significant regulators of tissue-damaging inflammatory responses in tuberculosis.
  • Targeting platelet-leukocyte interactions presents a promising host-directed therapeutic strategy for TB and potentially other lung diseases.