Related Experiment Video

Updated: May 13, 2026

Cell Membrane Repair Assay Using a Two-photon Laser Microscope
06:35

Cell Membrane Repair Assay Using a Two-photon Laser Microscope

Published on: January 2, 2018

A role for CASM in the repair of damaged Golgi architecture

Seeun Oh1, Saif Ullah1, Bhaskar Saha1,2

  • 1Department of Molecular Genetics and Microbiology, University of New Mexico Health Sciences Center, Albuquerque, NM, USA.

Autophagy
|May 12, 2026
PubMed

Insights

Cellular asymmetric membrane (CASM) response preserves Golgi apparatus integrity by coordinating lysosomal biogenesis and lipid conjugation, aiding cellular homeostasis after membrane damage.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Autophagy Research

Background:

  • The cellular asymmetric membrane (CASM) process involves lipid conjugation of Atg8-family proteins to damaged endomembranes, but its role in cellular homeostasis remains unclear.
  • TRIM46, a ubiquitin ligase, influences microtubule organization and Golgi apparatus structure.

Purpose of the Study:

  • To investigate the role of CASM in maintaining Golgi apparatus architecture, particularly after the loss of TRIM46.
  • To elucidate the mechanisms by which CASM contributes to cellular homeostasis in response to membrane damage.

Main Methods:

  • Utilized TRIM46-deficient cell models to observe Golgi morphology and Atg8-family protein localization.
  • Investigated the impact of genetic inhibition of CASM on TFEB activation and Golgi structure.
  • Assessed Golgi reformation capacity following drug-induced fragmentation in cells with reduced CASM gene expression.

Main Results:

  • TRIM46 deficiency led to Golgi ribbon fragmentation and enhanced TFEB-driven lysosomal biogenesis, with Atg8-family proteins localizing to the Golgi.
  • Golgi Atg8ylation in TRIM46-deficient cells mimicked CASM and was non-degradative.
  • Inhibiting CASM in TRIM46-deficient cells worsened Golgi defects and reduced TFEB activation, indicating CASM's role in Golgi repair.
  • Knockdown of CASM genes impaired Golgi reformation after drug-induced fragmentation.

Conclusions:

  • CASM is a crucial component of the Golgi damage response, acting to preserve Golgi integrity.
  • Lysosomal biogenesis and CASM are coordinated processes involved in repairing Golgi apparatus damage.
  • The study identifies a novel role for CASM in maintaining Golgi homeostasis.
Keywords:
Atg8ylationCASMVAILautophagygolgi damage/golgi fragmentationlysosomal biogenesistripartite motif

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