Related Experiment Video
Updated: May 13, 2026
11:51
Potato Virus X-Based microRNA Silencing (VbMS) In Potato.
Published on: May 11, 2020
MicroRNA858a antagonistically regulates plant response to concurrent biotic and abiotic stresses
Zheng Zhou1,2, Peiwu Li1,3, Daguang Cai2
1Biomanufacturing Institute, Xianghu Laboratory, Hangzhou, China.
Plant Signaling & Behavior
|May 12, 2026
Summary
Plant microRNA 858a (miR858a) is regulated by multiple stress-responsive transcription factors, revealing new insights into plant adaptation mechanisms. This discovery aids in developing crops with enhanced resistance to environmental challenges.
Area of Science:
- Plant Molecular Biology
- Plant Stress Physiology
- Gene Regulation
Background:
- Plants possess adaptive mechanisms to environmental stresses.
- MicroRNA 858a (miR858a) regulates plant responses to biotic and abiotic stresses by targeting transcription factor MYB111.
- The transcriptional regulation of miR858a remains largely unknown.
Purpose of the Study:
- To elucidate the transcriptional regulation of miR858a.
- To identify transcription factors (TFs) that bind to the miR858a promoter.
- To understand the role of these TFs in plant stress responses.
Main Methods:
- Yeast one-hybrid (Y1H) assay using the miR858a promoter and an Arabidopsis TF library.
- Analysis of TF expression profiles under flg22 and UV-B stress treatments.
- Prioritization of candidate TFs based on stress responsiveness.
Main Results:
- Identified 32 TFs that specifically interact with the miR858a promoter.
- Discovered five flg22-responsive TFs (ERF73, bZIP63, NF-YC6, AL7, AIN1) and one UV-B-responsive TF (MYBD).
- Demonstrated that miR858a is antagonistically regulated by distinct stress-responsive TFs.
Conclusions:
- miR858a's expression is modulated by a complex interplay of stress-responsive TFs.
- Highlights the critical role of miRNA regulation in plant adaptation to diverse environmental stresses.
- Provides insights for breeding crops with improved resistance to concurrent stresses.
More Related Videos
Related Concept Videos
01:09Microbe-Plant Interactions
Microbe-plant interactions represent a dynamic spectrum of associations shaped by intricate chemical signaling. These interactions can be neutral, beneficial, or detrimental, and profoundly influence plant physiology, growth, and ecosystem function. The plant microbiome, comprising bacteria, fungi, archaea, protists, and viruses, plays a pivotal role in mediating these effects through surface colonization, internal colonization, or systemic symbiosis.Mutualistic associations, particularly with...
01:25Cell Signaling in Plants
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
01:22MicroRNAs
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
01:22MicroRNAs
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
01:22MicroRNAs
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
01:56Riboswitches
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...