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Updated: May 13, 2026

Removal and Replacement of Endogenous Ligands from Lipid-Bound Proteins and Allergens
Published on: February 24, 2021
Combined glycosylation processing reduces immunoreactivity of litopenaeus vannamei matrix by modifying allergen
Meng Liu1, Xin-Yi Huang2, Qi-Fei Sun2
1School of Marine Biology, Applied Technology Engineering Center of Fujian Provincial Higher Education for Marine Food Nutrition Safety and Advanced Processing, Xiamen Ocean Vocational College, Xiamen, Fujian 361100, China; College of Ocean Food and Biological Engineering, Fujian Provincial Engineering Technology Research Center of Marine Functional Food, Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering, Jimei University, Xiamen, Fujian 361021, China.
Abstract:
The current study was designed to evaluate the influence of combined glycosylation processing modification on the immune binding activity and edible quality of the litopenaeus vannamei matrix. Three chemical processing methods had been compared, and the glycosylation processing could reduce the IgE/IgG-binding activity of TM to a large extent (25.8% ± 0.1% and 29.9% ± 2.4%, respectively) by destroying the structure and modifying lysine. Subsequently, the optimized combined glycosylation processing of shrimp matrix presented lower IgE-binding activity (75.97% ± 8.74%). As hypothesized, for the 4 important allergens in shrimp, high-frequency modifications of lysine (29.4%), asparagine (29.4%), and arginine (21.6%) were identified on the predicted epitopes. The processed shrimp meat exhibited better edible quality, including a more appealing appearance, better texture, and attractive flavor. In conclusion, the combined glycosylation processing showed low immunoreactivity and better quality in the shrimp matrix. This research provides a theoretical basis for the industrialization of hypoallergenic processing methods for shrimp.
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