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Pharmacogenomics: Identification of New Drug Targets

Advances in genomics have profoundly influenced drug discovery by increasing both the speed and accuracy of pharmaceutical development. Pharmacogenomics, which examines how genetic variation influences drug response, facilitates the identification of novel therapeutic targets and enables patient stratification for personalized treatment. These strategies contribute to improved drug efficacy, minimized adverse effects, and more efficient clinical trial design.Mapping genetic differences...

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Single-Cell Transcriptome-Wide Mendelian Randomization and Colocalization Uncover Potential Immunocytes-Related

Xingjian Zhang1, Yong Tian1, Xiaoying Ren1

  • 1Department of Endocrinology, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, People's Republic of China.

Journal of Cellular and Molecular Medicine
|May 12, 2026
PubMed
Summary

This study identifies immune cell genes causally linked to obesity using Mendelian randomization. It highlights potential new drug targets for obesity treatment derived from immune cell gene expression data.

Keywords:
Mendelian randomizationdrug target prioritizationimmune cellsobesitysingle‐cell eQTL

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Sample Preparation to Bioinformatics Analysis of DNA Methylation: Association Strategy for Obesity and Related Trait Studies
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Sample Preparation to Bioinformatics Analysis of DNA Methylation: Association Strategy for Obesity and Related Trait Studies

Published on: May 6, 2022

Area of Science:

  • Immunology
  • Genetics
  • Metabolic Disorders

Background:

  • Obesity complications necessitate effective weight-loss treatments.
  • The role of immune cells in obesity is increasingly recognized, but therapeutic targets remain elusive.
  • Identifying key genes linking immune cells and obesity is crucial for developing novel treatments.

Purpose of the Study:

  • To investigate causal relationships between immune cell expression quantitative trait loci (eQTL) and obesity-related traits.
  • To identify and prioritize novel, immune cell-specific gene targets for obesity therapy.
  • To validate findings using Mendelian randomization and colocalization analyses.

Main Methods:

  • Mendelian randomization (MR) analysis to assess causality between 14 immune cell eQTLs and obesity, body mass index, and body fat percentage.
  • Colocalization analysis to validate MR findings.
  • Pathway enrichment, differential gene expression (DEG) analysis, and drug-target prioritization using a Tier system.

Main Results:

  • 1630 genes showed evidence of a causal link with obesity-related traits, predominantly expressed in CD4+ naive/central memory T cells.
  • Antigen processing and presentation pathways were significantly enriched.
  • Forty-one genes were causally linked to all three obesity traits, with 19 being novel in the context of obesity. UBE2Z, ZCCHC7, and FNBP4 showed differential expression consistent with MR results. PYGB was identified as a top drug target.

Conclusions:

  • Immune cells play a significant role in obesity pathophysiology.
  • This study provides a prioritized list of potential cell-specific, immune-related gene targets for obesity treatment.
  • The findings offer new avenues for therapeutic strategies targeting immune mechanisms in obesity.