Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 13, 2026

Optimization of the Retinal Vein Occlusion Mouse Model to Limit Variability
07:23

Optimization of the Retinal Vein Occlusion Mouse Model to Limit Variability

Published on: August 6, 2021

Mechanical Stress Induces VEGF Expression and RPE Disruption in Mouse Eyes.

Akira Minamoto1, Ji-Ae Ko1, Kota Haruyama1

  • 1Department of Ophthalmology and Visual Science, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8551, Japan.

Biology
|May 12, 2026
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Fungal keratitis caused by a dematiaceous Pleosporales member following ocular trauma with a wooden fragment: a case report.

Journal of ophthalmic inflammation and infection·2026
Same author

Preoperative risk factors for uncontrolled intraocular pressure after vitrectomy combined with intrascleral fixation of the intraocular lens.

Japanese journal of ophthalmology·2026
Same author

Relationship between Corvis ST tonometry parameters and mean blur rate in the optic nerve head tissue area in healthy eyes.

PloS one·2026
Same author

Late-onset <i>Exophiala dermatitidis</i> keratitis after cataract surgery: A case report and literature review.

Medical mycology case reports·2026
Same author

Corvis ST parameters in primary open angle and angle closure glaucoma after propensity score matching.

Scientific reports·2026
Same author

Efficacy of Adalimumab Therapy for Non-Infectious Uveitis in Japanese Patients: Methotrexate Concomitance Across Different Uveitis Etiologies and the Effect of Dose Spacing.

Ocular immunology and inflammation·2026

Mechanical stress causes retinal pigment epithelium (RPE) damage and triggers angiogenic signaling in vivo. This study provides a model to explore stress-related retinal responses and anti-VEGF therapies.

Area of Science:

  • Ophthalmology
  • Cell Biology
  • Biomedical Engineering

Background:

  • Mechanical stress is linked to retinal pigment epithelium (RPE) dysfunction and angiogenesis in retinal diseases.
  • The direct in vivo impact of mechanical stress on the RPE-choroid complex is not fully understood.

Purpose of the Study:

  • To investigate the in vivo effects of mechanical stress on the RPE-choroid complex.
  • To establish a mouse model for studying mechanical stress-induced retinal responses.

Main Methods:

  • Localized mechanical stress was induced in mice via subconjunctival glass bead implantation.
  • Ocular tissues were analyzed using histological, immunohistochemical, and molecular methods.
  • Inflammatory mediators were quantified using multiplex cytokine assays.
Keywords:
VEGFmechanical stressmouse modelneovascular AMDretinal pigment epithelium

More Related Videos

A Mouse Model for Laser-induced Choroidal Neovascularization
07:08

A Mouse Model for Laser-induced Choroidal Neovascularization

Published on: December 27, 2015

Quantification of Vascular Parameters in Whole Mount Retinas of Mice with Non-Proliferative and Proliferative Retinopathies
12:28

Quantification of Vascular Parameters in Whole Mount Retinas of Mice with Non-Proliferative and Proliferative Retinopathies

Published on: March 12, 2022

Related Experiment Videos

Last Updated: May 13, 2026

Optimization of the Retinal Vein Occlusion Mouse Model to Limit Variability
07:23

Optimization of the Retinal Vein Occlusion Mouse Model to Limit Variability

Published on: August 6, 2021

A Mouse Model for Laser-induced Choroidal Neovascularization
07:08

A Mouse Model for Laser-induced Choroidal Neovascularization

Published on: December 27, 2015

Quantification of Vascular Parameters in Whole Mount Retinas of Mice with Non-Proliferative and Proliferative Retinopathies
12:28

Quantification of Vascular Parameters in Whole Mount Retinas of Mice with Non-Proliferative and Proliferative Retinopathies

Published on: March 12, 2022

Main Results:

  • Mechanical stress caused serous retinal detachment, photoreceptor outer segment elongation, and RPE tight junction (ZO-1) disruption.
  • VEGF expression and inflammatory mediators (MCP-1) were significantly upregulated in the RPE-choroid complex.
  • Anti-VEGF agents reduced stress-induced VEGF expression.

Conclusions:

  • Mechanical stress is sufficient to induce structural damage and angiogenic signaling in the RPE in vivo.
  • The developed mouse model is valuable for studying stress-related retinal conditions and VEGF-targeted therapies.