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Published on: June 1, 2017
Untargeted Metabolomics Reveals Metabolic Reprogramming Associated with Variable Temperature Stratification During
Manyi Fu1,2, Fengjuan Zhou2, Chang Liu1
1College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Biology
|May 12, 2026
Summary
Variable temperature stratification releases seed dormancy in Zanthoxylum armatum by altering jasmonic acid (JA) metabolism and lipid pathways. This metabolic reprogramming is key to overcoming dormancy and promoting germination.
Area of Science:
- Plant Physiology
- Metabolomics
- Seed Dormancy
Background:
- Deep physiological dormancy in Zanthoxylum armatum seeds hinders propagation.
- Variable temperature stratification is effective for dormancy release, but its metabolic underpinnings are unclear.
Purpose of the Study:
- To investigate the metabolic changes in Zanthoxylum armatum seeds during variable temperature stratification.
- To identify key metabolic pathways involved in dormancy release.
Main Methods:
- UPLC-MS/MS-based untargeted metabolomics.
- Multivariate statistical analyses (PCA, OPLS-DA).
- Variable temperature stratification (15°C then 4°C).
Main Results:
- 33 structurally annotated differential metabolites were identified.
- Significant alterations in α-linolenic acid and linoleic acid metabolism.
- Reduced levels of jasmonic acid (JA) and related oxylipins suggest JA pathway attenuation.
- Metabolic reprogramming involving phenylpropanoids, waxes, amino acids, and purines.
Conclusions:
- Variable temperature stratification induces extensive metabolic reprogramming in Zanthoxylum armatum seeds.
- Jasmonic acid-related lipid metabolism is a potential key pathway for dormancy release.
- Metabolic shifts support the transition from dormancy to germination.
