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Related Concept Videos

Protein-protein Interfaces02:04

Protein-protein Interfaces

Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a polypeptide...
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...

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Related Experiment Video

Updated: May 13, 2026

An Optimized Quantitative Pull-Down Analysis of RNA-Binding Proteins Using Short Biotinylated RNA
07:55

An Optimized Quantitative Pull-Down Analysis of RNA-Binding Proteins Using Short Biotinylated RNA

Published on: February 17, 2023

Emerging Technologies in RNA-Protein Interaction Analysis.

Nishinki T Muthumuni1,2, Jia Guo1,2

  • 1Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA.

Biology
|May 12, 2026
PubMed
Summary
This summary is machine-generated.

Profiling RNA-protein interactions (RPIs) is challenging but crucial for understanding gene regulation and disease. This review systematically overviews current methods, including RNA-centric, protein-centric, and imaging approaches, for mapping these vital interactions.

Keywords:
CLIP-seqCRISPR/Cas13RNA interactomeRNA-binding proteinsRNA–protein interactionsimaging-based approachesproximity labeling

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Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions
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Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions

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Identification of Footprints of RNA:Protein Complexes via RNA Immunoprecipitation in Tandem Followed by Sequencing (RIPiT-Seq)
09:26

Identification of Footprints of RNA:Protein Complexes via RNA Immunoprecipitation in Tandem Followed by Sequencing (RIPiT-Seq)

Published on: July 10, 2019

Related Experiment Videos

Last Updated: May 13, 2026

An Optimized Quantitative Pull-Down Analysis of RNA-Binding Proteins Using Short Biotinylated RNA
07:55

An Optimized Quantitative Pull-Down Analysis of RNA-Binding Proteins Using Short Biotinylated RNA

Published on: February 17, 2023

Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions
10:52

Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions

Published on: September 28, 2017

Identification of Footprints of RNA:Protein Complexes via RNA Immunoprecipitation in Tandem Followed by Sequencing (RIPiT-Seq)
09:26

Identification of Footprints of RNA:Protein Complexes via RNA Immunoprecipitation in Tandem Followed by Sequencing (RIPiT-Seq)

Published on: July 10, 2019

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • RNA-protein interactions (RPIs), mediated by RNA-binding proteins (RBPs), are critical for post-transcriptional gene regulation.
  • Dysregulation of RPIs is implicated in various diseases, including cancer and neurodegenerative disorders.
  • Current methods for profiling RPIs face challenges due to interaction transience, low affinity, and dynamic nature.

Purpose of the Study:

  • To provide a comprehensive overview of current methodologies for investigating RPIs.
  • To evaluate RNA-centric, protein-centric, and imaging-based strategies for RPI profiling.
  • To compare these methods based on resolution, sensitivity, specificity, and biological applicability.

Main Methods:

  • Systematic review of existing RPI profiling techniques.
  • Discussion of RNA-centric and protein-centric experimental strategies.
  • Evaluation of in situ imaging-based approaches for dynamic RPI analysis.

Main Results:

  • Identified and categorized diverse methodologies for RPI investigation.
  • Compared the strengths and limitations of different RPI profiling approaches.
  • Highlighted the utility of imaging techniques for resolving spatial-temporal dynamics.

Conclusions:

  • No single method is sufficient for comprehensive RPI mapping.
  • Integrative strategies are essential for constructing high-resolution, context-dependent RPI maps.
  • Understanding RPIs is vital for both physiological processes and disease pathogenesis.