TiO₂ Nanocomposite GelMA Film as Wound Dressing: Physicochemical, Structural, Mechanical Properties and Antibacterial...

Bacterial infections can delay wound healing and represent serious medical problems both in the hospital and community settings, especially skin wound infections caused by Staphylococcus aureus. In this work, a gelatin hydrogel modified with photo-cross-linkable methacrylamide groups at 10% concentration (GelMA10%), enriched with titanium dioxide nanoparticles (TiO₂NPs), and loaded with Neomycin sulphate was developed with the aim to realize a tissue for wound care with improved mechanical and antimicrobial properties. TiO₂ nanocomposite GelMA films with two concentrations of TiO₂NPs were characterized to assess physicochemical, structural and mechanical properties by scanning electron microscopy equipped with an energy-dispersive X-ray spectrometer (SEM/EDX), micro-computed tomography (micro-CT) and X-ray photoelectron spectroscopy (XPS). TiO₂ nanocomposite GelMA films showed a more compact structure, reduced pore sizes and a higher compressive modulus at the increasing concentration of TiO₂NPs. They were able to absorb and retain water for a prolonged time; however, no significant differences in the swelling degree at the increasing concentration of TiO₂NPs were observed. In vitro drug release and antibacterial activity against Staphylococcus aureus of TiO₂ nanocomposite GelMA film enriched with higher concentrations of TiO₂NPs, identified as a suitable candidate for wound healing, were investigated. Both GelMA10% and TiO₂ nanocomposite GelMA films loaded with drug exhibited a strong antibacterial action, whereas GelMA10% containing only TiO₂NPs did not show any antimicrobial properties.