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Published on: February 19, 2016
Fluorescence/surface-enhanced Raman scattering dual-mode sensor based on Ag/CQDs/ZIF-8 for human carboxylesterase 1
Ziyu Xu1, Jinru Cao2, Ying Wang1
1Guangxi Key Laboratory of Green Processing of Sugar Resources, Department of Medicine, College of Biological and Chemical Engineering, Guangxi University of Science and Technology, Liuzhou, Guangxi, 545006, PR China.
Mikrochimica Acta
|May 12, 2026
Summary
A novel dual-mode sensor using Ag/CQDs/ZIF-8 nanocomposites enables sensitive detection of human carboxylesterase 1 (hCE1) in serum. This advancement aids in diagnosing diseases like cancer by accurately measuring hCE1 levels.
Area of Science:
- Biomaterials Science
- Analytical Chemistry
- Biomedical Engineering
Background:
- Human carboxylesterase 1 (hCE1) is a key biomarker for diseases, including cancer, found in liver, intestines, and tumors.
- Accurate measurement of hCE1 in serum is crucial for disease diagnosis and assessment.
- Existing detection methods may lack the sensitivity or specificity required for complex biological samples.
Purpose of the Study:
- To develop a sensitive and reliable dual-mode sensor for detecting hCE1 in human serum.
- To utilize Ag/CQDs/ZIF-8 nanocomposites for enhanced sensing capabilities.
- To combine fluorescence (FL) and surface-enhanced Raman scattering (SERS) for improved accuracy.
Main Methods:
- Preparation of Ag/CQDs/ZIF-8 nanocomposites incorporating silver nanoparticles and carbon quantum dots.
- Development of a dual-mode FL/SERS sensor using the nanocomposite.
- Application of an enzyme-linked immunosorbent assay (ELISA) with benzidine as the signal molecule.
Main Results:
- The dual-mode sensor achieved low limits of detection (LOD) of 0.1 ng·mL⁻¹ (FL) and 0.072 ng·mL⁻¹ (SERS).
- High recovery rates (92.44%–117.47%) indicate accurate quantification.
- The sensor demonstrated excellent selectivity, stability, and suitability for complex biological matrices.
Conclusions:
- The developed Ag/CQDs/ZIF-8 based dual-mode sensor offers a promising platform for sensitive hCE1 detection.
- This sensor enhances accuracy and reliability in quantitative measurements for clinical diagnostics.
- The technology holds significant potential for detecting various biomolecules in clinical samples.
