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Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

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Nanozymes in Point-of-Care Testing: Classification, Biosensing Strategies, and Integrated Applications.

Wei Liu¹, Zhongwei Jiang¹, Xue Gong¹

¹Chongqing Key Laboratory of Green Catalysis Materials and Technology, College of Chemistry and Materials Science, Chongqing Normal University, Chongqing, PR China.

Chemical Record (New York, N.Y.)

|May 12, 2026

Summary

Nanozymes, artificial enzyme mimics, offer high stability for advanced biosensors. This review explores their classification, mechanisms, and applications in point-of-care testing (POCT) for biomarker detection.

Keywords:

nanozymes point‐of‐care testing recognition units signal amplifiers signal converters

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Area of Science:

Biotechnology
Nanomaterials Science
Analytical Chemistry

Background:

Nanozymes, artificial enzyme mimics, combine enzyme catalytic activity with nanomaterial stability.
They are crucial in biosensing and disease therapy.
Recent advancements focus on nanozyme integration into point-of-care testing (POCT) biosensors.

Purpose of the Study:

To systematically review recent advances in nanozyme-based POCT biosensors.
To analyze nanozyme classification, mechanisms of action, and applications.
To provide insights into design strategies and future directions for nanozyme-integrated POCT.

Main Methods:

Classification of nanozymes.
Analysis of nanozyme mechanisms in biomolecular recognition, signal conversion, and amplification.
Review of innovative applications in POCT for biomarker detection.

Main Results:

Nanozymes function as recognition units, signal converters, and signal amplifiers in POCT biosensors.
Detailed analysis of design strategies and functional positioning of nanozymes.
Discussion of application potential in detecting diverse biomolecules.

Conclusions:

Nanozyme-based POCT offers significant potential for rapid and sensitive biomarker detection.
Understanding nanozyme roles is key to designing effective POCT systems.
Future research should address challenges and explore new applications.