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Related Concept Videos

Sample Preparation for Analysis: Overview01:21

Sample Preparation for Analysis: Overview

Sample preparation is an essential step in the analytical process. It involves preparing a sample so that it can be analyzed accurately. The goal is to extract the analyte, the substance you want to measure, from the sample while removing any components that may interfere with the analysis. Sample preparation techniques vary depending on the physical state of the sample.
Bulk or large solid samples are typically reduced in size using grinding, crushing, or milling techniques to increase the...

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Related Experiment Video

Updated: May 14, 2026

Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis
11:25

Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis

Published on: July 11, 2014

Practical guide for marine exo-metabolomic sample preparation.

Catherine C Bannon1, Jana K Geuer1, Lennart Stock2

  • 1Department of Symbiosis, Max Planck Institute for Marine Microbiology, Bremen, 28359, Germany.

The ISME Journal
|May 12, 2026
PubMed
Summary
This summary is machine-generated.

Marine metabolomics studies exo-metabolites, crucial for ocean ecosystems. This guide reviews methods for analyzing these compounds, aiding researchers in selecting optimal techniques for marine sample analysis.

Keywords:
dissolved organic materialenvironmental metabolomicsseawater

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Sample Preparation for Metabolic Profiling using MALDI Mass Spectrometry Imaging
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Last Updated: May 14, 2026

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Sample Preparation for Metabolic Profiling using MALDI Mass Spectrometry Imaging
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Sample Preparation for Metabolic Profiling using MALDI Mass Spectrometry Imaging

Published on: December 22, 2020

Area of Science:

  • Marine biology
  • Environmental science
  • Analytical chemistry

Background:

  • Exo-metabolites significantly influence marine microbial communities and global nutrient cycles.
  • Studying marine exo-metabolites is challenging due to low concentrations, high salinity, and chemical diversity.
  • Recent advancements offer improved methods for marine metabolomics.

Purpose of the Study:

  • Provide a practical guide for selecting marine exo-metabolomic extraction methods.
  • Systematically review five recently published methods for marine exo-metabolomics.
  • Assist researchers in choosing methods aligned with study goals and capabilities.

Main Methods:

  • Systematic review of five marine exo-metabolomic methods.
  • Focus on workflows for primary metabolite quantification.
  • Utilized liquid and gas chromatography coupled to mass spectrometry (LC-MS, GC-MS).

Main Results:

  • Highlighted challenges in marine metabolomics: low concentrations, high salt, and diversity.
  • Presented a selection guide based on research needs and experimental design.
  • Detailed practical constraints and sample handling for each reviewed method.

Conclusions:

  • The guide supports researchers in choosing appropriate marine exo-metabolomic methods.
  • Emphasizes the need for specialized techniques in marine metabolomics.
  • Identifies opportunities for future innovation in the field.