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Related Experiment Video

Updated: May 14, 2026

Sexual Crosses with the Mucoromycete Phycomyces blakesleeanus
05:34

Sexual Crosses with the Mucoromycete Phycomyces blakesleeanus

Published on: June 6, 2025

Mating-type locus structure affects gene expression in unidirectional mating-type switching fungi.

Frances A Lane1, Brenda D Wingfield1, Michael J Wingfield1

  • 1Department of Biochemistry, Genetics and Microbiology, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Private Bag X20, Hatfield 0028, South Africa.

G3 (Bethesda, Md.)
|May 12, 2026
PubMed
Summary
This summary is machine-generated.

Mating-type (MAT) locus structure in fungi influences gene expression beyond sexual reproduction. Even genetically identical isolates show distinct expression patterns based on MAT gene presence and arrangement.

Keywords:
Fungal 2026filamentous fungigene regulation networkshomothallism

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Area of Science:

  • Mycology
  • Genetics
  • Molecular Biology

Background:

  • Filamentous ascomycetes exhibit diverse mating behaviors, including self-fertility and self-sterility.
  • Some species undergo unidirectional mating-type switching, altering their sexual compatibility.
  • The mating-type (MAT) locus, particularly MAT1-1 and MAT1-2 genes, controls sexual reproduction and can be structurally modified during switching.

Purpose of the Study:

  • To investigate how variations in the MAT1 locus structure affect gene expression in the filamentous fungus Ceratocystis albifundus.
  • To determine if MAT1 locus structure influences genes unrelated to the sexual cycle.

Main Methods:

  • Comparative transcriptomic analysis was performed on three genetically identical isolates of Ceratocystis albifundus.
  • Isolates included one MAT-2 self-fertile, one MAT-1 self-sterile, and one MAT-2 self-sterile type.
  • Gene expression profiles were analyzed focusing on MAT genes, pheromone-receptor pathways, and other cellular functions.

Main Results:

  • Distinct gene expression patterns were observed across the three MAT1 locus variants.
  • Differences were noted in the expression of mating-type genes and components of the pheromone-receptor pathway.
  • Significant variations in gene expression were also found for genes not directly associated with mating processes.

Conclusions:

  • The structure of the MAT1 locus has a broader impact on fungal gene expression than previously understood.
  • MAT1 locus variations influence not only sexual reproduction but also other cellular pathways.
  • Understanding MAT1 locus structure is crucial for comprehending fungal development and behavior beyond mating.