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Extracellular space in some isolated tissues.

D J McIver, A D Macknight

    The Journal of Physiology
    |May 1, 1974
    PubMed
    Summary
    This summary is machine-generated.

    Inulin and polyethylene glycol (PEG) 4000 serve as extracellular markers in toad bladder cells. However, inulin and PEG 1000 can enter mammalian cell water, affecting extracellular space measurements.

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    Area of Science:

    • Physiology
    • Biochemistry

    Background:

    • Determining extracellular space is crucial for understanding cellular transport and function.
    • Inulin, polyethylene glycol (PEG) of varying molecular weights, and sucrose are commonly used markers, but their suitability can vary by tissue type.

    Purpose of the Study:

    • To evaluate the effectiveness of inulin, PEG 4000, PEG 1000, and sucrose as extracellular space markers in mammalian kidney and liver slices and toad bladder epithelial cells in vitro.

    Main Methods:

    • Incubation of mammalian tissue slices and toad bladder epithelial cells with isotopically labeled inulin, PEG 4000, PEG 1000, and sucrose.
    • Analysis of marker distribution within cellular and extracellular compartments.
    • Extraction efficiency of markers from dried and undried tissue samples.

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    Main Results:

    • Inulin could not be fully extracted from dried mammalian tissue, suggesting cellular entry, but was fully recovered from toad bladder cells.
    • PEG 4000 occupied a similar space to inulin in dried mammalian tissue.
    • PEG 1000 and sucrose entered cellular water in mammalian slices.
    • PEG 1000 behaved as an extracellular marker in toad bladder cells, similar to inulin.

    Conclusions:

    • Inulin acts as an extracellular marker in toad bladder cells but enters mammalian cell water.
    • PEG 4000 is a reliable extracellular marker for both mammalian and toad bladder preparations.
    • PEG 1000 is suitable for toad bladder cells but not mammalian slices.
    • Sucrose is not a reliable extracellular marker in either tissue type due to cellular entry.