Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

MALDI-TOF Mass Spectrometry

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...

Matrix-Assisted Laser Desorption Ionization (MALDI)

Matrix-Assisted Laser Desorption Ionization (MALDI)

Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI is an ionization technique, widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix...

Mass Analyzers: Overview

Mass Analyzers: Overview

The mass analyzer is a crucial component of the mass spectrometer. In the ionization chamber, the vaporized sample is bombarded with a high-energy electron beam to generate a radical cation and further fragment into neutral molecules, radicals, and cations. A series of negatively charged accelerator plates accelerate the cations into the mass analyzer. The mass analyzer separates ions according to their mass-to-charge (m/z) ratios and then directs them to the detector. The common types of mass...

High-Resolution Mass Spectrometry (HRMS)

High-Resolution Mass Spectrometry (HRMS)

The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For example, the mass of helium...

Mass Spectrum: Interpretation

Mass Spectrum: Interpretation

An unknown compound can be established by identifying the molecular ion peak in the mass spectrum. The molecular ion peak is often weak or absent due to the predominance of fragmentation in high-energy electron beams. In such cases, a soft-energy electron beam can be used to scan the spectrum to enhance the intensity of the molecular ion peak. Additionally, chemical ionization, field ionization, and desorption ionization spectra are used to obtain a relatively intense molecular ion peak.To...

Mass Spectrometers

Mass Spectrometers

This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

GPC3-specific dnTGFβRII-armoured CAR T cells for hepatocellular carcinoma.

Nature·2026

Same author

Programming Multidomain Peptides With Molecular Frustration Into Biomolecular Condensates.

Small (Weinheim an der Bergstrasse, Germany)·2026

Same author

Atomic-Level Polishing of Single-Crystal Diamond Using a Combination of Reactive Ion Etching and Chemical Mechanical Polishing.

Materials (Basel, Switzerland)·2026

Same author

Cardiac-Derived ECM Microspheres for Enhanced hiPSC-CMs Maturation.

Advanced functional materials·2026

Same author

Functional Design of Near-Infrared Aggregation-Induced Emission Photosensitizers Targeting Cell Membrane and Mitochondria Enhance Photodynamic Efficiency.

Advanced healthcare materials·2026

Same author

Intrapericardially injected hydrogel loaded with stromal cell secretome microparticles improves post-infarction myocardial repair in pigs.

European heart journal·2026

Search research articles

Related Experiment Video

Updated: May 14, 2026

Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization (IR-MALDESI)

Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization (IR-MALDESI)

Published on: March 24, 2016

A High-Voltage Floating MALDI Source for Ultra-High-Resolution Mass Spectrometer.

Zhengge Chen¹, Yi Hong², Yixue Zhao¹

¹College of Environment and Climate, Institute of Mass Spectrometry and Atmospheric Environment, Jinan University, Guangzhou, China.

Rapid Communications in Mass Spectrometry : RCM

|May 12, 2026

Summary

We optimized an intermediate-pressure MALDI source for high-voltage operation, enabling seamless integration with planar electrostatic ion traps. This advancement mitigates electrical discharge risks for improved ion source performance.

Keywords:

IP‐MALDI discharge high voltage structural design

More Related Videos

Molecular Beam Mass Spectrometry With Tunable Vacuum Ultraviolet (VUV) Synchrotron Radiation

Molecular Beam Mass Spectrometry With Tunable Vacuum Ultraviolet (VUV) Synchrotron Radiation

Published on: October 30, 2012

Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer

Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer

Published on: November 26, 2013

Related Experiment Videos

Last Updated: May 14, 2026

Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization (IR-MALDESI)

Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization (IR-MALDESI)

Published on: March 24, 2016

Molecular Beam Mass Spectrometry With Tunable Vacuum Ultraviolet (VUV) Synchrotron Radiation

Molecular Beam Mass Spectrometry With Tunable Vacuum Ultraviolet (VUV) Synchrotron Radiation

Published on: October 30, 2012

Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer

Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer

Published on: November 26, 2013

Area of Science:

Analytical Chemistry
Mass Spectrometry Instrumentation

Background:

High-resolution planar electrostatic ion traps (PEIT) require 4 keV ion injection energy.
Integrating high-voltage (HV) MALDI sources into intermediate-pressure (IP) environments poses electrical discharge risks.

Purpose of the Study:

To structurally optimize an IP-MALDI source for safe HV floating operation.
To ensure seamless coupling with a PEIT instrument.

Main Methods:

SIMION simulations were used to optimize pressure and voltage parameters for ion transmission.
Physical architecture, including chamber and exhaust tube geometries, was refined to mitigate discharge risks.
The optimized source was integrated and experimentally validated with the PEIT.

Main Results:

Simulations showed optimal performance at 10 Pa, enabling 5 kV operation with mitigated discharge risks.
The integrated system demonstrated a detectable mass-to-charge (m/z) range of 89-9500.
Successful experimental validation confirmed the system's feasibility and efficacy.

Conclusions:

An IP-MALDI source was developed for stable HV floating operation, meeting PEIT coupling requirements.
This provides a robust solution for operating IP ion sources at high potentials.
Facilitates broader application and advancement of IP ion source technology.