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Preparation of Food Samples Using Homogenization and Microwave-Assisted Wet Acid Digestion for Multi-Element Determination with ICP-MS
Published on: December 22, 2023
Evaluating the essential microminerals in chicken and beef from Java, Indonesia using neutron activation analysis for
Diah Dwiana Lestiani1, Syukria Kurniawati2, Woro Yatu Niken Syahfitri2
1Research Center for Nuclear Beam Analysis Technology, Research Organization for Nuclear Energy, National Research and Innovation Agency (BRIN), BJ Habibie Science and Technology Area, Serpong, Indonesia. diah003@brin.go.id.
Abstract:
Iron, selenium, and zinc are crucial for growth, immune function, and overall health, particularly in early childhood, where deficiencies can lead to nutritional problems such as stunting, a major health issue in Indonesia. This study measured the concentrations of these minerals in chicken and beef using neutron activation analysis (NAA). A total of 146 samples were collected from 73 traditional markets across five provinces in Java: Jakarta, West Java, Central Java, East Java and Yogyakarta. Samples were irradiated for 2 h at a thermal neutron flux of 1013 n·cm-2·s-1 with the rabbit system at the G.A. Siwabessy Reactor, Serpong, and analyzed using a high-purity germanium (HPGe) gamma spectrometer. On a wet-weight basis, chicken contained of 5.85 ± 2.59 µg/g iron, 0.19 ± 0.14 µg/g selenium, and 12.3 ± 7.19 µg/g zinc, while in beef were 15.75 ± 8.66 µg/g, 0.20 ± 0.18 µg/g, and 37.45 ± 16.11 µg/g, respectively. Dietary intake estimates, calculated from national consumption data that contribution from chicken and beef were below the recommended dietary allowance (RDA) for all three microminerals, with a highest of 18.2% for selenium in adult males. This study provides valuable data for updating the Indonesian Food Composition Table and highlight the application of neutron activation analysis as a reliable tool for food nutrient characterization in Indonesia.
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