Maturity-Dependent Volatile Flavor Profiling of Baked Potatoes via HS-SPME-GC-MS, Multivariate Statistical Analysis,...

This study explored the flavor profiling of baked potatoes, with a focus on how maturity affects the volatile flavor. By using HS-SPME-GC-MS, sensory evaluation, multivariate statistical analysis and computational modeling, a total of 99 volatile compounds were finally identified. Multivariate statistical analysis yielded 36 different important compounds (VIP > 1, p < 0.05). Subsequently, combined with relative odor activity value (ROAV), four key compounds including 2-ethyl-3,5-dimethylpyrazine, 2,6-diethylpyrazine, ethyl acetate and benzeneacetaldehyde were identified as potential indicators of baked potatoes with different maturities. Further, molecular docking analysis revealed the interactions between key pyrazine compounds and human olfactory receptors OR5K1 through hydrogen bonds and other interactions. These findings provide new insights into the relationship between potato maturity and flavor differences, and also lays a foundation for in-depth exploration into flavor identification and perception.