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Analysis of Interactions between Endobiotics and Human Gut Microbiota Using In Vitro Bath Fermentation Systems
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Structural Characterization and In Vitro Fermentation Properties of Polysaccharides from Polygonatum filipes.

Huimin Hu1,2, Jiawei Wang1,2, Kaijun Wang1,2

  • 1College of Food and Health, Zhejiang Agriculture and Forestry University, Hangzhou 311300, China.

Foods (Basel, Switzerland)
|May 13, 2026
PubMed
Summary

A novel fructan polysaccharide, PFP-80, isolated from Polygonatum filipes, promotes gut health by increasing beneficial bacteria and inhibiting pathogens. This study supports PFP-80 as a functional food ingredient.

Keywords:
Polygonatum filipesSCFAsin vitro fermentationpolysaccharides

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Area of Science:

  • Phytochemistry
  • Microbiology
  • Nutritional Science

Background:

  • Polysaccharides from medicinal plants are increasingly recognized for their health benefits.
  • Polygonatum species are traditional herbs with various bioactive compounds.
  • Understanding the structure and function of plant-derived polysaccharides is crucial for developing functional foods.

Purpose of the Study:

  • To isolate and characterize a homogeneous polysaccharide (PFP-80) from Polygonatum filipes root.
  • To investigate the effects of PFP-80 on gut microbiota fermentation and composition.
  • To evaluate the potential of PFP-80 as a functional food ingredient.

Main Methods:

  • Enzymatic extraction and ethanol precipitation were used to isolate PFP-80.
  • Gas chromatography-mass spectrometry (GC-MS) and nuclear magnetic resonance (NMR) were employed for structural analysis.
  • In vitro fermentation assays were conducted to assess PFP-80's impact on gut microbiota.

Main Results:

  • PFP-80 was identified as a fructan polysaccharide (4.06 kDa) with specific β-D-Fruf linkages and branching at the O-6 position.
  • Fermentation of PFP-80 led to decreased pH and significantly increased short-chain fatty acids (SCFAs).
  • PFP-80 enhanced gut microbial abundance and diversity while inhibiting the growth of pathogens like Ruminococcus gnavus.

Conclusions:

  • PFP-80 is a structurally defined fructan polysaccharide with prebiotic properties.
  • PFP-80 positively modulates gut microbiota composition and function.
  • These findings provide a scientific foundation for developing PFP-80 into functional food products.