Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mitochondrial integrated stress response activation creates a therapeutic vulnerability to MCL-1 inhibition in acute myeloid leukemia.

Cell death & disease·2026
Same author

Author Correction: cBAF complex components and MYC cooperate early in CD8<sup>+</sup> T cell fate.

Nature·2026
Same author

Control of naive T cell reactivity and peripheral tolerance by ascorbate and TET activity.

Science advances·2026
Same author

Large-scale, spatially resolved panoramic CRISPR screening in native tissue environments using Perturb-DBiT.

Nature biotechnology·2026
Same author

ATG7 Limits Basal Antiviral Gene Expression and Moderately Promotes VSV Replication in Mammalian Non-Immune Cells.

Pathogens (Basel, Switzerland)·2026
Same author

IRS4 is a PI3K-activating cancer dependency up-regulated through DNA rearrangements or epigenetic mechanisms in multiple solid tumors.

Science advances·2026

Related Experiment Video

Updated: May 14, 2026

CRISPR/Cas9-mediated Targeted Integration In Vivo Using a Homology-mediated End Joining-based Strategy
08:22

CRISPR/Cas9-mediated Targeted Integration In Vivo Using a Homology-mediated End Joining-based Strategy

Published on: March 12, 2018

Essential HDRescue: A Co-Targeting Strategy to Enhance Precision Genome Editing by Co-Editing Essential Genes.

Jamaica F Siwak1,2,3, Jon P Connelly1,2, Shondra M Pruett-Miller1,2,3,4

  • 1Center for Advanced Genome Engineering, St. Jude Children's Research Hospital, Memphis, TN 38103, USA.

Cells
|May 13, 2026
PubMed
Summary

This study introduces Essential HDRescue, a novel genome editing method that enhances precise DNA modifications. It boosts homology-directed repair (HDR) efficiency by co-targeting essential genes, simplifying cell isolation and reducing costs.

Keywords:
CRISPRHDRcell line developmentco-targetingessential genesgenome engineering

More Related Videos

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

Genome Editing and Directed Differentiation of hPSCs for Interrogating Lineage Determinants in Human Pancreatic Development
09:37

Genome Editing and Directed Differentiation of hPSCs for Interrogating Lineage Determinants in Human Pancreatic Development

Published on: March 5, 2017

Related Experiment Videos

Last Updated: May 14, 2026

CRISPR/Cas9-mediated Targeted Integration In Vivo Using a Homology-mediated End Joining-based Strategy
08:22

CRISPR/Cas9-mediated Targeted Integration In Vivo Using a Homology-mediated End Joining-based Strategy

Published on: March 12, 2018

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

Genome Editing and Directed Differentiation of hPSCs for Interrogating Lineage Determinants in Human Pancreatic Development
09:37

Genome Editing and Directed Differentiation of hPSCs for Interrogating Lineage Determinants in Human Pancreatic Development

Published on: March 5, 2017

Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • Genome editing is crucial but faces challenges with efficiency and cell selection.
  • Homology-directed repair (HDR) is often suppressed by non-homologous end-joining (NHEJ) in mammalian cells.
  • Current methods for isolating edited cells are laborious and costly.

Purpose of the Study:

  • To develop a novel enrichment method, Essential HDRescue, to increase HDR efficiency.
  • To improve the isolation of cells with precise genome modifications.
  • To overcome the limitations of current genome editing workflows.

Main Methods:

  • Co-targeting an essential genomic locus alongside the desired target site.
  • Utilizing intrinsic positive and negative selection at the essential gene locus.
  • Applying the Essential HDRescue method in cancer cell lines and induced pluripotent stem cells (iPSCs).

Main Results:

  • Essential HDRescue significantly increased HDR rates without exogenous reporters or drugs.
  • Achieved up to a 6-fold increase in single-allele edits and a 4-fold increase in homozygous edits.
  • Demonstrated a widely applicable method for enhancing precise genome editing outcomes.

Conclusions:

  • Essential HDRescue harnesses intrinsic cellular dependencies for improved genome editing.
  • The method offers a cost-effective and efficient solution for precise genetic modifications.
  • This approach facilitates the generation of edited cells with minimal scars at essential genes.