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Microbiota of the Urogenital Tract01:28

Microbiota of the Urogenital Tract

The human urogenital system, once thought to be sterile in healthy individuals, is now recognized as a complex microbial habitat. Advancements in molecular sequencing techniques have revealed that even in healthy adults, the kidneys and bladder harbor microbial populations similar to those found in the distal urethra, albeit in much lower abundance. These resident microorganisms, while generally innocuous, can become opportunistic pathogens under conditions that alter the urogenital...
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The human body harbors a vast and diverse viral community known as the human virome. The virome includes bacteriophages that infect bacteria, and eukaryotic viruses that infect human cells. Transient dietary and environmental viruses also contribute to this dynamic ecosystem. Estimates suggest the human body may contain on the order of 10¹³ viral particles, though abundance varies widely by body site and detection method.Comprehensive characterization of the virome has become possible only with...
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Vagina01:26

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Correction: Luca et al. Global and Regional Diagnostic Results of Progress Toward Cervical Cancer Elimination, According to the WHO Strategy: A Systematic Literature Review with Narrative Synthesis. <i>Diagnostics</i> 2026, <i>16</i>, 1224.

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Updated: May 14, 2026

Collection, Isolation, and Flow Cytometric Analysis of Human Endocervical Samples
12:34

Collection, Isolation, and Flow Cytometric Analysis of Human Endocervical Samples

Published on: July 6, 2014

Vaginal Microbiota Composition and HPV Genotype-Specific CIN2+ Risk: A Cross-Sectional Study.

Alexandru Hamod1, Razvan Popovici1, Mihaela Oancea2

  • 1Grigore T. Popa University of Medicine and Pharmacy, 700115 Iasi, Romania.

Diagnostics (Basel, Switzerland)
|May 13, 2026
PubMed
Summary
This summary is machine-generated.

Vaginal microbiome markers, like Lactobacillus depletion, can improve cervical cancer screening by predicting CIN2+ outcomes beyond current tests. This approach may be most beneficial for HPV-16-negative women.

Keywords:
CINteccervical intraepithelial neoplasiahuman papillomavirusmicrobiotarisk stratification

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Last Updated: May 14, 2026

Collection, Isolation, and Flow Cytometric Analysis of Human Endocervical Samples
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Published on: July 6, 2014

Determination of Biofilm Initiation on Virus-infected Cells by Bacteria and Fungi
12:33

Determination of Biofilm Initiation on Virus-infected Cells by Bacteria and Fungi

Published on: July 6, 2016

Area of Science:

  • Gynecology
  • Microbiome research
  • Cervical cancer screening

Background:

  • Vaginal microbiome dysbiosis is linked to HPV persistence and cervical intraepithelial neoplasia (CIN) progression.
  • The added prognostic value of microbiome markers beyond existing triage assays is not well-established.

Purpose of the Study:

  • To assess if Lactobacillus depletion and Shannon diversity improve CIN2+ and CIN3+ prediction beyond CINtec and HPV-16 genotyping.
  • To evaluate the incremental discriminative performance of microbiome markers in cervical cancer screening.

Main Methods:

  • Secondary analysis of a cross-sectional study with 82 women undergoing cervical screening.
  • Modified Poisson regression to estimate associations with CIN2+, CIN3+, and CINtec positivity.
  • Assessed incremental predictive value using AUC, DeLong's test, NRI, and IDI.

Main Results:

  • HPV-16 was the strongest predictor for CIN2+ and CIN3+.
  • Lactobacillus depletion showed significant prognostic value in HPV-16-negative women.
  • Microbiome markers significantly improved CIN2+ prediction when added to CINtec + HPV-16, particularly for non-events.

Conclusions:

  • Vaginal microbiome markers, especially Lactobacillus depletion, offer significant incremental value for predicting CIN2+.
  • Microbiome-based triage may enhance cervical cancer screening effectiveness, particularly in HPV-16-negative individuals.