Related Experiment Video
Updated: May 14, 2026
10:09
A Straightforward Method for Glucosinolate Extraction and Analysis with High-pressure Liquid Chromatography (HPLC)
Published on: March 15, 2017
Transcriptome Analysis Reveals Intensity-Dependent Regulation of UV-B Radiation on Glucosinolate Biosynthesis in
Pengpeng Mao1, Song Chen1, Le Kong2
1College of Agriculture, Nanjing Agricultural University, Nanjing 211800, China.
Plants (Basel, Switzerland)
|May 13, 2026
Summary
Low-intensity UV-B radiation significantly boosts glucosinolate levels in rapeseed leaves by upregulating key biosynthetic genes. High-intensity UV-B had an opposite effect, highlighting UV-B
Area of Science:
- Plant Science
- Biochemistry
- Agricultural Science
Background:
- Rapeseed ('double-low' cultivars) has low glucosinolate levels in vegetative tissues.
- Glucosinolates are important compounds in Brassica species.
- UV-B radiation's effect on glucosinolates in rapeseed is not fully understood.
Purpose of the Study:
- To investigate if UV-B radiation can enhance glucosinolate accumulation in rapeseed.
- To examine the modulation of glucosinolate profiles and biosynthetic pathways under different UV-B intensities.
Main Methods:
- Rapeseed seedlings ('double-low' cultivar NY4) were exposed to white light with low-intensity UV-B (UVBL) or high-intensity UV-B (UVBH).
- Glucosinolate content and profiles were analyzed in leaves after 21 days of treatment.
- Transcriptome analysis was performed to identify changes in gene expression related to glucosinolate biosynthesis.
Main Results:
- UVBL significantly increased total glucosinolate content by 64.57%, mainly due to progoitrin and neoglucobrassicin.
- UVBH decreased total glucosinolates but increased gluconasturtiin.
- UVBL upregulated key genes (e.g., MAM, IPMDH, CYP79F1, SOT17/18) and transcription factors (e.g., MYB28, MYB34, MYB51, MYB122) involved in glucosinolate biosynthesis.
Conclusions:
- Low-intensity UV-B radiation effectively enhances total glucosinolate content in rapeseed leaves.
- UV-B radiation modulates glucosinolate profiles and associated gene expression.
- Transcriptional reprogramming is a key mechanism underlying UV-B-induced glucosinolate accumulation.
Related Concept Videos
01:23Regulation of Expression at Multiple Steps
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the addition of a...
01:57Adaptations that Reduce Water Loss
Though evaporation from plant leaves drives transpiration, it also results in loss of water. Because water is critical for photosynthetic reactions and other cellular processes, evolutionary pressures on plants in different environments have driven the acquisition of adaptations that reduce water loss.
01:29Translational Regulation
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
01:56Riboswitches
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
01:10Transcription
Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...