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Related Concept Videos

Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the addition of a...

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Updated: May 14, 2026

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
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Regulation of Tau Alternative Splicing: A Novel Role for the Ribonucleoprotein RBM20.

Andrea Corsi1, Angela Valentino1, Maria Giusy Bruno1

  • 1Department of Neuroscience, Biomedicine and Movement Sciences, University of Verona, 37134 Verona, Italy.

International Journal of Molecular Sciences
|May 13, 2026
PubMed
Summary
This summary is machine-generated.

Researchers identified RNA-binding motif protein 20 (RBM20) as a key regulator of Tau exon splicing. This finding advances understanding of neurodegenerative diseases like Alzheimer's and highlights brain organoids for studying Tau pathologies.

Keywords:
MAPTPTBP1RBM20Taualternative splicing

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Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay

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Last Updated: May 14, 2026

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Published on: October 9, 2014

Using the E1A Minigene Tool to Study mRNA Splicing Changes
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Using the E1A Minigene Tool to Study mRNA Splicing Changes

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Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay
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Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay

Published on: August 26, 2018

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Genetics

Background:

  • Tau protein is crucial for neuronal cytoskeleton stability and axonal transport.
  • Dysregulation of Tau splicing and hyperphosphorylation are implicated in tauopathies, including Alzheimer's disease.
  • Understanding Tau exon splicing mechanisms is vital for neurodegenerative disorder research.

Purpose of the Study:

  • To investigate the role of specific splicing factors in regulating Tau exon expression.
  • To utilize cell lines and neuronal organoids to study Tau splicing regulation.
  • To explore Tau expression dynamics during cerebral organoid differentiation.

Main Methods:

  • Application of RNA-binding assays to study protein-RNA interactions.
  • Quantitative Polymerase Chain Reaction (qPCR) for precise gene expression analysis.
  • Utilizing cell lines and 3D cerebral organoid models for biological investigation.

Main Results:

  • The splicing factor RNA-binding motif protein 20 (RBM20) was identified as a regulator of Tau exon 6 and exon 10 expression.
  • Tau expression patterns were observed to be regulated during cerebral organoid differentiation, mirroring in vivo development.
  • The study confirmed the feasibility of using brain organoids to study Tau alternative splicing in neural development.

Conclusions:

  • RBM20 plays a significant role in controlling Tau alternative splicing.
  • Cerebral organoid models effectively recapitulate in vivo Tau expression during neural development.
  • 3D cellular models offer a viable platform for studying Tau splicing and related pathologies.