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Related Concept Videos

Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

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Related Experiment Video

Updated: May 14, 2026

Development of Mouse-Derived Organoid Lines from Fallopian Tube Epithelial Cells for High Grade Serous Ovarian Carcinoma Modeling
06:51

Development of Mouse-Derived Organoid Lines from Fallopian Tube Epithelial Cells for High Grade Serous Ovarian Carcinoma Modeling

Published on: August 6, 2025

Signaling Pathway Dissection After Progesterone Receptor Enhancement in an Immortalized Pre-Cancer Fallopian Tube

Yu-Hsun Chang1, Kun-Chi Wu2, Dah-Ching Ding3,4

  • 1Department of Pediatrics, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Tzu Chi University, Hualien 970, Taiwan.

International Journal of Molecular Sciences
|May 13, 2026
PubMed
Summary

Progesterone receptor (PR) isoforms A and B have distinct roles in fallopian tube epithelial cells (FTECs). Understanding these differences in PR-A and PR-B signaling is key for ovarian cancer research.

Keywords:
FE25fallopian tube epithelial cellsp53progesterone receptorsretinoblastoma

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Ex Vivo Culture of Primary Human Fallopian Tube Epithelial Cells
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Ex Vivo Culture of Primary Human Fallopian Tube Epithelial Cells

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An Efficient Method for Extracting Human Fallopian Tube Epithelia for Single-cell Analyses
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An Efficient Method for Extracting Human Fallopian Tube Epithelia for Single-cell Analyses

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Related Experiment Videos

Last Updated: May 14, 2026

Development of Mouse-Derived Organoid Lines from Fallopian Tube Epithelial Cells for High Grade Serous Ovarian Carcinoma Modeling
06:51

Development of Mouse-Derived Organoid Lines from Fallopian Tube Epithelial Cells for High Grade Serous Ovarian Carcinoma Modeling

Published on: August 6, 2025

Ex Vivo Culture of Primary Human Fallopian Tube Epithelial Cells
11:46

Ex Vivo Culture of Primary Human Fallopian Tube Epithelial Cells

Published on: May 9, 2011

An Efficient Method for Extracting Human Fallopian Tube Epithelia for Single-cell Analyses
07:03

An Efficient Method for Extracting Human Fallopian Tube Epithelia for Single-cell Analyses

Published on: March 28, 2025

Area of Science:

  • Gynecologic Oncology
  • Molecular Endocrinology
  • Cellular Biology

Background:

  • Ovarian cancer, particularly high-grade serous carcinoma (HGSC), is a lethal malignancy with poor survival rates, often diagnosed at advanced stages.
  • HGSC is thought to originate from fallopian tube epithelial cells (FTECs), influenced by hormonal signaling.
  • Progesterone receptor (PR) expression, especially PR-B, correlates with better HGSC prognosis, but isoform-specific functions in precancerous FTECs are unknown.

Purpose of the Study:

  • To investigate the distinct biological and transcriptomic effects of Progesterone Receptor-A (PR-A) and Progesterone Receptor-B (PR-B) in p53- and Rb-defective FTEC-derived cells (FE25).

Main Methods:

  • Stable overexpression of PR-A and PR-B in FE25 cells.
  • Cell proliferation, apoptosis, and senescence assays.
  • Progesterone (P4) treatment and analysis of cell-cycle arrest and apoptosis.
  • RNA sequencing to analyze transcriptomic changes.
  • Analysis of downstream signaling pathways (PI3K-Akt, MAPK).

Main Results:

  • PR-A suppressed proliferation, enhanced apoptosis, and induced senescence.
  • PR-B promoted proliferation and activated JNK/c-Jun signaling.
  • Both isoforms mediated cell-cycle arrest and apoptosis upon P4 treatment, with PR-A showing stronger Sub-G1 induction.
  • PR-A and PR-B differentially regulated cell-cycle inhibitors and senescence markers.
  • RNA sequencing revealed broader P4-induced transcriptomic changes with PR-B, affecting immune, angiogenic, and proliferative programs.

Conclusions:

  • PR-A and PR-B exhibit distinct and complementary roles in FTEC biology and response to progesterone.
  • These isoform-specific effects in FE25 cells provide mechanistic insights but are context-dependent and not direct predictors of clinical outcomes.