Related Experiment Video
Updated: May 14, 2026

Experimental Protocol of a Three-minute, All-out Arm Crank Exercise Test in Spinal-cord Injured and Able-bodied Individuals
Published on: June 8, 2017
Effects of Dietary Nitrate and Caffeine on End Power and Work Above End Power During a 3 min All-Out Test in Trained
Anthony M Hagele1, Kyle L Sunderland1, Petey W Mumford2
1Exercise and Performance Nutrition Laboratory, College of Science, Technology, and Health, Lindenwood University, St. Charles, MO 63301, USA.
Background:
The purpose of this study was to examine the effects of acute dietary nitrate (NO3-) and caffeine (CAF) supplementation on end power (EP) and work performed above EP (WEP) in trained male cyclists during a 3 min all-out test (3MT) on a cycle ergometer.
Methods:
Fifteen healthy, trained male cyclists (28.5 ± 5.3 years, 79.2 ± 9.1 kg, VO2peak 55.2 ± 5.6 mL·kg-1·min-1) completed four exercise trials in a randomized, double-blind, placebo-controlled, crossover study design separated by 3-7 days. The four experimental conditions were placebo beverage (nitrate-depleted) + placebo capsule, nitrate-rich beetroot juice + placebo capsule (BR), placebo beverage + caffeine capsule (CAF), and nitrate-rich beetroot juice + caffeine capsule (BR + CAF). Participants consumed nitrate-rich beetroot juice (~13 mmol NO3-) or nitrate-depleted placebo three hours before exercise, and caffeine (5 mg∙kg-1) or maltodextrin placebo one hour before testing. EP and WEP were determined from the 3MT. Secondary outcomes included peak and mean power output. Data were analyzed using a repeated-measures ANOVA with repeated measures on condition. A p-value of 0.05 was used to determine statistical significance. Effect size was evaluated using partial eta squared.
Results:
No significant effect of condition was observed for EP (p = 0.401, ηp2 = 0.056), WEP (p = 0.580, ηp2 = 0.048), peak power (p = 0.642, ηp2 = 0.046), mean power (p = 0.212, ηp2 = 0.108), or total work (p = 0.217, ηp2 = 0.107).
Conclusions:
No statistically significant differences between conditions were detected under the conditions of the present study.
More Related Videos
07:26Conducting Maximal and Submaximal Endurance Exercise Testing to Measure Physiological and Biological Responses to Acute Exercise in Humans
Published on: October 17, 2018
09:33Using Near-Infrared Spectroscopy Wearable Devices to Identify Central Versus Peripheral Limitations During Exercise
Published on: December 19, 2024
Related Concept Videos
Antianginal Drugs: Nitrates and β-Blockers
Organic nitrates, such as nitroglycerin, play a pivotal role. Once metabolized, they liberate nitric oxide, a molecular marvel. Nitric oxide triggers guanylyl cyclase and augments cGMP production. This biochemical cascade orchestrates the relaxation of vascular smooth muscles, ushering in vasodilation and enhancing coronary blood flow. Administered...
Exercise and Muscle Performance
Endurance exercises
Endurance exercises involve running, swimming, or cycling, which require repetitive movements with low force output. When a person engages in endurance exercise, a few noticeable changes occur in their skeletal muscles. For instance, the number of capillaries...
Exercise and Cardiac Output
Sustained exercise increases the muscles' oxygen demand, which can be met...
Nitric Oxide Signaling Pathway
Exercise and Cardiovascular Response
Light to moderate physical activity initiates a series of interconnected responses in the body. The heart rate modestly increases in anticipation of the workout, followed by widespread vasodilation as oxygen consumption by skeletal muscles increases. This results in decreased peripheral resistance, increased capillary blood flow, and accelerated...
Muscle Recovery and Fatigue