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Hydroponic System: A Novel, Rapid Greenhouse Inoculation Method for Wheat Crown Rot
Lefan Pu1, Qiaojun Jin1, Jianxin Guo1
1State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education, College of Plant Protection, Northwest A&F University, Xianyang, Shaanxi 712100, P.R. China.
A new hydroponic method improves wheat crown rot (CR) inoculation efficiency. This integrated system, combining disease index and fungal biomass, offers precise and reliable resistance assessment for Fusarium pseudograminearum.
Area of Science:
- Plant Pathology
- Agricultural Science
- Molecular Biology
Background:
- Crown rot (CR), caused by Fusarium pseudograminearum (Fp), significantly impacts global wheat production.
- Current methods for CR inoculation and resistance assessment are inefficient, inconsistent, and lack precision.
Purpose of the Study:
- To develop an efficient hydroponic inoculation method for wheat seedlings.
- To create an integrated system for precise assessment of wheat CR resistance.
Main Methods:
- Hydroponic inoculation of wheat seedlings at 24°C with 5 × 10^6 conidia/ml Fp.
- Integrated resistance assessment combining refined disease index (DI) and in planta fungal biomass quantification (fungal DNA/wheat DNA).
Main Results:
- Hydroponic inoculation reduced experimental duration from 21-45 to 18 days.
- The integrated system demonstrated precision in assessing resistance across five wheat varieties.
- Varieties showed varying resistance levels: Sunco (near resistant), Mingxian169 (moderately resistant), Xiaoyan22, Fielder, Xinong979 (highly susceptible).
Conclusions:
- The developed hydroponic method and integrated assessment system enhance efficiency, consistency, and precision in CR research.
- These advancements facilitate large-scale germplasm screening for wheat CR resistance.
- The system aids in advancing research on F. pseudograminearum-wheat interactions.
