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Related Concept Videos

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...
Inheritance of Chromatin Structures03:17

Inheritance of Chromatin Structures

Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying DNA...
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
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Non-LTR Retrotransposons03:18

Non-LTR Retrotransposons

As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
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RNA Interference

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Related Experiment Video

Updated: May 14, 2026

Isolation of Group 2 Innate Lymphoid Cells from Mouse Nasal Mucosa to Detect the Expression of CD226
08:30

Isolation of Group 2 Innate Lymphoid Cells from Mouse Nasal Mucosa to Detect the Expression of CD226

Published on: May 10, 2022

The long non-coding RNA Dreg1 is required for optimal ILC2 development.

Sara Quon1,2, Adelynn Tang3,4,5, Nadia Iannarella1,2

  • 1The Walter and Eliza Hall Institute of Medical Research, Parkville, Australia.

Elife
|May 13, 2026
PubMed
Summary
This summary is machine-generated.

The non-coding RNA Dreg1 is crucial for the development of group 2 innate lymphoid cells (ILC2s). Its absence selectively reduces ILC2 numbers by impacting Gata3 expression in early progenitors.

Keywords:
chromosomesgene expressiongene regulationhumanimmune developmentimmunologyinflammationinnate lymphoid cellslong non-coding RNAmouse

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Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR
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Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR

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Related Experiment Videos

Last Updated: May 14, 2026

Isolation of Group 2 Innate Lymphoid Cells from Mouse Nasal Mucosa to Detect the Expression of CD226
08:30

Isolation of Group 2 Innate Lymphoid Cells from Mouse Nasal Mucosa to Detect the Expression of CD226

Published on: May 10, 2022

Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR
13:04

Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR

Published on: March 1, 2019

Area of Science:

  • Immunology
  • Developmental Biology
  • Transcriptional Regulation

Background:

  • Gata3 is a key transcription factor for immune cell development, including T cells, NK cells, and ILCs.
  • Precise regulation of Gata3 expression is vital for immune cell lineage commitment.
  • The Gata3 locus contains complex regulatory elements, including distal enhancers.

Purpose of the Study:

  • To investigate the function of the non-coding RNA Dreg1, located near the Gata3 locus.
  • To determine Dreg1's role in immune cell development, particularly ILC2s.
  • To elucidate the regulatory mechanisms involving Dreg1 in Gata3 expression.

Main Methods:

  • Genetic manipulation in mice to excise the Dreg1 locus.
  • Analysis of immune cell populations (T cells, NK cells, ILCs) in Dreg1-deficient mice.
  • Chromatin profiling (H3K27ac) and gene expression analysis.
  • Investigation of Tcf1-dependent regulation of Dreg1.
  • Comparative analysis of Dreg1 homologues in human ILC2s.

Main Results:

  • Selective reduction of group 2 ILCs (ILC2s) in Dreg1-deficient mice, with other immune lineages unaffected.
  • Increased common innate lymphoid cell progenitors (ILCPs) and decreased ILC2 progenitors (ILC2Ps) in bone marrow.
  • Evidence of an early developmental bottleneck affecting Gata3 levels in progenitors.
  • Dreg1 locus accessibility in early progenitors and H3K27ac decoration in an ILCP-specific, Tcf1-dependent manner.
  • Dreg1 expression and its homologues are conserved in human ILC2s.

Conclusions:

  • Dreg1 is a Tcf1-dependent non-coding RNA essential for optimal ILC2 development.
  • Dreg1 plays a critical role in fine-tuning Gata3 expression levels required for ILC2 lineage commitment.
  • Dreg1 acts early in development to ensure sufficient Gata3 levels for ILC2 progenitors.