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Home
Programmable Circular Single-stranded Dna Acts As Recyclable Anti-mirna Nucleotides To Inhibit Colorectal Cancer.

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Programmable Circular Single-stranded Dna Acts As Recyclable Anti-mirna Nucleotides To Inhibit Colorectal Cancer.

Related Experiment Video

Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells

Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells

Published on: May 30, 2025

Programmable Circular Single-Stranded DNA Acts as Recyclable Anti-miRNA Nucleotides to Inhibit Colorectal Cancer.

Jinghao Wang^1,2, Pengfei Zhang^2,3,4, Guang Hu^2,5

¹Department of Chemistry, University of Science & Technology of China, Hefei, Anhui, China.

|May 13, 2026

View abstract on PubMed

Summary

This summary is machine-generated.

This study introduces circular single-stranded DNA (CssDNA) as a novel platform for colorectal cancer (CRC) therapy. CssDNA effectively targets multiple oncomiRs, restoring tumor suppressor gene expression and offering a stable, recyclable treatment option.

Keywords:

DNA‐based nanomedicines anti‐miRNA nucleotide circular single‐stranded DNA microRNA multi‐targeted therapy

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MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

Published on: October 7, 2025

Area of Science:

Biochemistry
Molecular Biology
Oncology

Background:

MicroRNA (miRNA) therapy shows promise for colorectal cancer (CRC) treatment.
Targeting single oncomiRs has limited efficacy due to simultaneous action of multiple oncomiRs in CRC.
Current anti-miRNA nucleotide (AMiN) designs face challenges like high synthetic error rates and difficulty in synthesizing long oligonucleotides.

Purpose of the Study:

To develop a novel, stable, and recyclable multi-oncomiR targeting platform for colorectal cancer (CRC) therapy.
To utilize circular single-stranded DNA (CssDNA) as a superior alternative to conventional AMiNs.
To demonstrate the efficacy of CssDNA in degrading multiple oncomiRs and restoring tumor suppressor gene (TSG) expression.

Main Methods:

Development of a multi-oncomiR targeting platform using in vivo M13 phage-generated circular single-stranded DNA (CssDNA).

CssDNA designed to act as a sponge, attracting and degrading multiple overexpressed oncomiRs in CRC.

Assessment of CssDNA's stability, low immunogenicity, recyclability, and efficacy in promoting TSG expression.

Main Results:

Engineered CssDNA demonstrated high stability, low immunogenicity, and potential for long oligonucleotide synthesis.
CssDNA effectively sponged and degraded multiple oncogenic miRNAs (oncomiRs) in colorectal cancer models.
Degradation of oncomiRs led to the restoration of tumor suppressor gene (TSG) expression, inhibiting CRC progression.
Released CssDNA exhibited recyclability, enabling a second round of therapeutic action.

Conclusions:

Circular single-stranded DNA (CssDNA) presents a promising, stable, and recyclable platform for multi-oncomiR targeting in colorectal cancer (CRC) therapy.
This novel CssDNA-based approach offers advantages over traditional anti-miRNA nucleotide (AMiN) designs.
The sequence-customizable CssDNA platform holds potential for antagonizing miRNA-mediated repression of tumor suppressor genes in various cancer treatments.