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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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Updated: May 14, 2026

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions
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In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

Published on: October 21, 2022

Circular RNA Circ_0020236 Inhibits Hepatocellular Carcinoma Cell Proliferation and Migration Through Modulating the

Jing Liu1, Bing Sun2, Peng Cao2

  • 1Guizhou University of Traditional Chinese Medicine, Guiyang, 550001, Guizhou, China.

Biochemical Genetics
|May 13, 2026
PubMed
Summary

Circular RNAs (circRNAs) like circ_0020236 inhibit hepatocellular carcinoma (HCC) progression by sponging miR-1825 and targeting IKBKB. This pathway, regulated by ESRP2, offers a potential therapeutic strategy for HCC.

Keywords:
Circ_0020236Hepatocellular carcinomaIKBKBMiR-1825Proliferation

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Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Hepatocellular carcinoma (HCC) is a highly aggressive malignancy with poor prognosis.
  • Circular RNAs (circRNAs) are implicated in HCC pathogenesis and progression.
  • The specific role of circ_0020236 in HCC remains largely unexplored.

Purpose of the Study:

  • To investigate the function and mechanism of circ_0020236 in hepatocellular carcinoma (HCC) progression.
  • To elucidate the regulatory axis involving circ_0020236, miR-1825, and IKBKB in HCC.
  • To identify potential therapeutic targets for HCC intervention.

Main Methods:

  • Quantitative real-time PCR to assess expression levels of circ_0020236, miR-1825, and IKBKB in HCC samples and cell lines.
  • Bioinformatics, dual-luciferase reporter, RNA immunoprecipitation, and RNA pull-down assays to validate molecular interactions.
  • In vitro (CCK-8, colony formation, Transwell) and in vivo assays to evaluate the functional roles of circ_0020236, miR-1825, and IKBKB in HCC cell proliferation, migration, and tumor growth.

Main Results:

  • circ_0020236 and IKBKB were downregulated, while miR-1825 was upregulated in HCC tissues and cells.
  • Overexpression of circ_0020236 suppressed HCC cell proliferation, migration, and tumor growth.
  • circ_0020236 acted as a molecular sponge for miR-1825, which targets IKBKB. Rescue experiments confirmed this interaction.
  • ESRP2 was identified as a positive regulator of circ_0020236 biogenesis.

Conclusions:

  • The ESRP2/circ_0020236/miR-1825/IKBKB axis plays a crucial role in inhibiting HCC progression.
  • circ_0020236 exhibits tumor-suppressive properties in HCC.
  • circ_0020236 represents a promising therapeutic target for hepatocellular carcinoma treatment.