Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Responses to Drought and Flooding02:41

Responses to Drought and Flooding

Water plays a significant role in the life cycle of plants. However, insufficient or excess of water can be detrimental and pose a serious threat to plants.
Plant Hormones01:56

Plant Hormones

Plant hormones—or phytohormones—are chemical molecules that modulate one or more physiological processes of a plant. In animals, hormones are often produced in specific glands and circulated via the circulatory system. However, plants lack hormone-producing glands.
Plant Hormones01:56

Plant Hormones

Plant hormones—or phytohormones—are chemical molecules that modulate one or more physiological processes of a plant. In animals, hormones are often produced in specific glands and circulated via the circulatory system. However, plants lack hormone-producing glands.
Regulation of Transpiration by Stomata02:04

Regulation of Transpiration by Stomata

During photosynthesis, plants acquire the necessary carbon dioxide and release the produced oxygen back into the atmosphere. Openings in the epidermis of plant leaves is the site of this exchange of gasses. A single opening is called a stoma—derived from the Greek word for “mouth.” Stomata open and close in response to a variety of environmental cues.
Gene Regulation During Sporulation01:17

Gene Regulation During Sporulation

Sporulation is a complex developmental process that allows certain Gram-positive bacteria, such as Bacillus subtilis and Clostridium species, to survive extreme environmental conditions. This process is tightly regulated by a series of signaling cascades and transcriptional controls, ensuring the formation of a highly resistant endospore.Sporulation is triggered by unfavorable conditions, such as nutrient depletion, and is governed by a phosphorelay system. One of the sensor kinases, such as...
Adaptations that Reduce Water Loss01:57

Adaptations that Reduce Water Loss

Though evaporation from plant leaves drives transpiration, it also results in loss of water. Because water is critical for photosynthetic reactions and other cellular processes, evolutionary pressures on plants in different environments have driven the acquisition of adaptations that reduce water loss.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Osteoporosis and Fracture Risk Associated with Novel Antidepressants: A Systematic Review and Meta-Analysis.

Actas espanolas de psiquiatria·2024
Same author

Epidemiologic features and therapeutic strategies of kerion: A nationwide multicentre study.

Mycoses·2024
Same author

A Coppoborylene Stabilized by Multicenter Covalent Bonding and Its Amphoteric Reactivity to CO.

Angewandte Chemie (International ed. in English)·2024
Same author

Protein Signature Differentiating Neutrophils and Myeloid-Derived Suppressor Cells Determined Using a Human Isogenic Cell Line Model and Protein Profiling.

Cells·2024
Same author

Adsorption of Multiple NO Molecules on Anionic Gold Clusters: Electron Transfer and Disproportionation Enhanced by Molecular Aggregation.

The journal of physical chemistry. A·2024
Same author

MORN motif-containing protein OsMORN1 and OsMORN2 are crucial for rice pollen viability and cold tolerance.

The Plant journal : for cell and molecular biology·2024

Related Experiment Video

Updated: May 14, 2026

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput
10:29

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput

Published on: March 30, 2018

OsSAP3 Enhances Rice Seed Vigor by Modulating Hydrogen Peroxide Homeostasis.

Qingshan Mu1,2, Yihan Liu1,2, Xuefan Wu2

  • 1Hainan Institute, Zhejiang University, Sanya, 572000, Hainan, China.

Rice (New York, N.Y.)
|May 13, 2026
PubMed
Summary

The stress-associated protein gene OsSAP3 is crucial for rice seed vigor and temperature adaptability. Its regulation impacts germination, reserve mobilization, and response to abscisic acid (ABA) and hydrogen peroxide (H2O2).

Keywords:
OsSAP3ABAROSReserve mobilizationSeed vigor

More Related Videos

Development of a Cabbage Protoplast System for Studying Hypoxia Tolerance in Brassica
08:09

Development of a Cabbage Protoplast System for Studying Hypoxia Tolerance in Brassica

Published on: September 20, 2024

Pattern-Triggered Oxidative Burst and Seedling Growth Inhibition Assays in Arabidopsis thaliana
04:11

Pattern-Triggered Oxidative Burst and Seedling Growth Inhibition Assays in Arabidopsis thaliana

Published on: May 21, 2019

Related Experiment Videos

Last Updated: May 14, 2026

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput
10:29

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput

Published on: March 30, 2018

Development of a Cabbage Protoplast System for Studying Hypoxia Tolerance in Brassica
08:09

Development of a Cabbage Protoplast System for Studying Hypoxia Tolerance in Brassica

Published on: September 20, 2024

Pattern-Triggered Oxidative Burst and Seedling Growth Inhibition Assays in Arabidopsis thaliana
04:11

Pattern-Triggered Oxidative Burst and Seedling Growth Inhibition Assays in Arabidopsis thaliana

Published on: May 21, 2019

Area of Science:

  • Plant Biology
  • Molecular Genetics
  • Agricultural Science

Background:

  • Seed vigor is essential for successful rice germination and establishment.
  • The molecular mechanisms regulating rice seed vigor are not fully understood.
  • Stress-associated protein genes play roles in plant stress responses.

Purpose of the Study:

  • To identify key regulators of rice seed vigor.
  • To elucidate the role of OsSAP3 in seed germination and vigor.
  • To understand how OsSAP3 influences responses to abscisic acid (ABA) and hydrogen peroxide (H2O2).

Main Methods:

  • Quantitative reverse transcription PCR (qRT-PCR) to analyze OsSAP3 expression.
  • Generation and analysis of OsSAP3 knockout rice mutants (Ossap3).
  • Measurement of reserve mobilization, ABA signaling gene expression, and H2O2 levels.
  • Exogenous application of H2O2 to evaluate its effect on Ossap3 seed vigor.

Main Results:

  • OsSAP3 is highly expressed during rice seed development and germination, induced by ABA.
  • Ossap3 mutants exhibit reduced reserve mobilization, elevated ABA signaling, and decreased seed vigor.
  • Ossap3 seeds have a narrower optimal temperature range for germination due to lower H2O2 levels.
  • Exogenous H2O2 application restored seed vigor in Ossap3 by modulating ABA signaling and enhancing starch mobilization.

Conclusions:

  • OsSAP3 is a key regulator of rice seed vigor, influencing germination, reserve mobilization, and ABA sensitivity.
  • OsSAP3-mediated regulation of H2O2 levels is critical for temperature adaptability during germination.
  • Understanding OsSAP3's role provides insights into enhancing rice seed quality and crop establishment.