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Updated: May 14, 2026

Apical Resection Mouse Model to Study Early Mammalian Heart Regeneration
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A Modified Neonatal Mouse Cardiac Apicoectomy Model: Reproducibility and High Survival Rates.

Jiahao Ren1, Shudan Xiao1, Kun Wang1

  • 1Department of Cardiovascular Surgery, Institute of Chronic Diseases, The Affiliated Hospital of Qingdao University, Qingdao, China.

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
|May 13, 2026
PubMed
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This study refines the neonatal mouse heart apical resection (AR) model for cardiac regeneration research. Optimized cryoanesthesia and thoracotomy techniques significantly improve survival rates and model stability.

Area of Science:

  • Cardiovascular Research
  • Regenerative Medicine
  • Neonatal Physiology

Background:

  • Neonatal mouse heart apical resection (AR) is a model for studying cardiac regeneration.
  • Existing AR models lack standardized parameters, affecting reproducibility.
  • Precise control over cryoanesthesia, temperature, and surgical approach is needed.

Purpose of the Study:

  • To optimize the neonatal mouse heart apical resection (AR) model.
  • To establish precise parameters for cryoanesthesia duration, ambient temperature, and thoracotomy site.
  • To improve survival rates and reproducibility in cardiac regeneration studies.

Main Methods:

  • Varied cryoanesthesia duration, ambient temperature, and thoracotomy location.
  • Quantified blood loss and survival rates.
Keywords:
cardiac regenerationhigh survival ratesneonatal mouse heart apical resection

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Last Updated: May 14, 2026

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  • Utilized RT-qPCR, immunofluorescence, and Masson staining to analyze cardiac repair.

    • Main Results:

    • A protocol of 4 min 30 s cryoanesthesia with a 10-min delay at 24-28°C ensured cardiac arrest and a stable operative window.
    • A fifth-intercostal thoracotomy approach minimized bleeding and improved survival.
    • The refined AR protocol consistently induced injury, transient dysfunction, and regeneration.

    Conclusions:

    • Optimized parameters for cryoanesthesia and thoracotomy enhance the neonatal mouse heart AR model.
    • The refined protocol provides a stable and reproducible platform for studying neonatal cardiac regeneration.
    • This improved model facilitates research into mechanisms of cardiac repair in early life.