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Published on: April 23, 2019
Leveraging micro-disk array device for sensitive duloxetine detection in human urine.
Antonia Kalagia1, Marianna Ntorkou2, Constantinos K Zacharis2
1Laboratory of Analytical Chemistry, Department of Chemistry, Aristotle University of Thessaloniki, GR-54124, Greece; Laboratory of Pharmaceutical Analysis, Department of Pharmacy, Aristotle University of Thessaloniki, GR-54124, Greece.
A novel microdisk array (MDA) technique efficiently quantifies duloxetine in urine using HPLC-FLD. This reusable, miniaturized method offers enhanced mass transfer, reduced solvent use, and demonstrates excellent accuracy and precision.
Area of Science:
- Analytical Chemistry
- Separation Science
- Biomedical Analysis
Background:
- Quantification of duloxetine in biological matrices is crucial for therapeutic drug monitoring.
- Existing sample preparation methods can be time-consuming, solvent-intensive, and less efficient.
- There is a need for miniaturized, reusable, and sustainable extraction techniques.
Purpose of the Study:
- To develop and validate a microdisk array (MDA)-based microextraction technique for duloxetine quantification.
- To assess the efficiency, reusability, and environmental impact of the novel MDA system.
- To apply the developed method for the analysis of duloxetine in human urine samples.
Main Methods:
- Development of a reusable microdisk array (MDA) device using C18-coated microdisks on a stainless-steel spinal needle.
- Optimization of adsorption and desorption parameters for duloxetine extraction from aqueous samples.
- Quantification of duloxetine using High-Performance Liquid Chromatography with a Fluorescence Detector (HPLC-FLD).
Main Results:
- The MDA method achieved a linear response for duloxetine in the range of 25-500 ng/mL with a limit of detection of 15 ng/mL.
- High precision (intra- and inter-day variability <3.1%) and accuracy (relative recovery 102.4%-118.1%) were obtained.
- The MA index confirmed the low environmental impact, and the method was successfully applied to human urine samples.
Conclusions:
- The developed MDA-based microextraction technique is an efficient, miniaturized, and reusable sample preparation strategy.
- The method offers significant advantages in terms of reduced sample manipulation and solvent consumption.
- The MDA approach presents a sustainable and viable alternative for the analysis of duloxetine in biological fluids.
