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Tuning complexation and functionality of lactoferrin-low methoxyl pectin complexes by chlorogenic acid during
Wenfei Fu1, Yuxin He1, Guo Tian1
1Department of Food Science, College of Food Science and Engineering, Jilin University, Changchun 130062, China.
None:
The interactions between chlorogenic acid (CA) and lactoferrin (LF)-low methoxyl pectin (LMP) electrostatic complexes were examined during gradual acidification starting from an initial alkaline condition (pH 10). CA modulated the physicochemical and functional properties of LF-LMP complexes, particularly at pH range of 5-8. CA reduced the hydrodynamic diameter of the LF-LMP complexes and decreased the intrinsic fluorescence intensity and surface hydrophobicity, whereas surface hydrophobicity increased with rising pH. No significant changes in the secondary structure were observed. LF-LMP-CA exhibited enhanced emulsifying activity, emulsifying stability, and foam stability, as well as improved DPPH and ABTS radical scavenging capacities compared to LF-LMP. In addition, none of the complexes showed apparent cytotoxic effects on the Caco-2 cells under the conditions tested. These resulted indicated that CA can modulate the complexation behavior, structure, and functionality of LF-LMP complexes. These findings provide a basis for the development of protein-polysaccharide-polyphenol complexes in food systems.
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