Related Experiment Video
Updated: May 15, 2026
Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings
Published on: February 3, 2021
[Development and application of amplification-free nucleic acid detection in molecular diagnostics]
1Shanghai Songjiang Clinical Medical College of Nanjing Medical University, Nanjing 211166, China Department of Clinical Laboratory, Songjiang Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 201600, China.
None:
Amplification-free nucleic acid detection (AF-NAD) is a highly sensitive analytical approach that enables the detection of nucleic acids in samples without the need for amplification. This article reviews three categories of AF-NAD technologies: single-molecule detection technology, enzyme-catalyzed reaction-based detection technology, and hybridization capture-based detection technology, with an emphasis on the performance characteristics of each technology and their applications in fields such as rapid diagnosis of infectious diseases, precision oncology, early screening of genetic diseases, and point-of-care testing (POCT) for public health emergencies. Additionally, the advantages and disadvantages of these technologies are discussed, and their development trends and research directions are explored, providing a reference for the development of faster, simpler, more sensitive, efficient, and low-contamination detection technologies.
