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Related Experiment Video

Updated: May 15, 2026

An Effective Inoculation Method for Phytophthora capsici on Black Pepper Plants
08:58

An Effective Inoculation Method for Phytophthora capsici on Black Pepper Plants

Published on: September 16, 2022

Pepper Veinal Mottle Virus (PVMV)-Based Vector for Expressing Pathogen-Derived Resistance in Pepper.

Changhui Deng1, Na Li1, Hongguang Cui2

  • 1Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests (Ministry of Education) and School of Tropical Agriculture and Forestry, Hainan University, Haikou, China.

Methods in Molecular Biology (Clifton, N.J.)
|May 13, 2026
PubMed
Summary
This summary is machine-generated.

Pathogen-derived resistance (PDR) offers effective plant virus management. This study details a novel virus-based vector protocol for delivering viral coat protein (CP) to trigger PDR in pepper plants, overcoming genetic transformation challenges.

Keywords:
Coat proteinGolden Gate cloningPathogen-derived resistancePepper veinal mottle virusPepper virusesViral vector

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Last Updated: May 15, 2026

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Published on: February 27, 2021

Area of Science:

  • Plant pathology
  • Molecular biology
  • Agricultural biotechnology

Background:

  • Pathogen-derived resistance (PDR) is a key strategy for managing plant viral diseases in agriculture.
  • Genetically modified crops expressing viral coat protein (CP) demonstrate efficacy against specific viruses.
  • Cultivated pepper species present significant challenges for genetic transformation and regeneration.

Purpose of the Study:

  • To develop an alternative method for inducing PDR in pepper plants.
  • To utilize an attenuated virus-based vector for delivering target viral genes.
  • To establish a protocol for triggering PDR in pepper against viral pathogens.

Main Methods:

  • Employing an attenuated strain of pepper veinal mottle virus (PVMV) as a vector.
  • Designing the vector for the delivery of target virus CP or genomic fragments.
  • Developing a detailed protocol for vector application in pepper plants.

Main Results:

  • Successful delivery of viral CP into pepper plants using the PVMV-based vector.
  • Demonstration of PDR induction in treated pepper plants.
  • Establishment of a viable alternative to genetic transformation for PDR.

Conclusions:

  • The PVMV-based vector system provides a practical approach for PDR induction in recalcitrant pepper varieties.
  • This method offers a promising strategy for enhancing crop resistance to viral diseases.
  • The protocol facilitates the application of PDR in agricultural settings for pepper cultivation.