Pepper Veinal Mottle Virus (PVMV)-Based Vector for Expressing Pathogen-Derived Resistance in Pepper

Pathogen-derived resistance (PDR) is an effective approach in the management of plant viruses in agriculture. It is achieved via expressing a viral protein or genomic fragment in a host plant. Numerous genetically modified crops expressing viral coat protein (CP) show good performance in defending target viruses. Unfortunately, the cultivated pepper species are recalcitrant to genetic transformation and regeneration. As an alternative, an attenuated virus-based vector can be used for the delivery of desired viral gene or genomic fragment into host plant to trigger PDR. Here, we have described in detail a protocol using an attenuated strain of pepper veinal mottle virus (PVMV)-based vector for the delivery of target virus CP in pepper, thus triggering PDR.