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Updated: May 15, 2026

TurboID-Based Proximity Labeling for In Planta Identification of Protein-Protein Interaction Networks
Published on: May 17, 2020
A TurboID-Based Protocol for Efficient Interacting Proteins Identification Using Proximity Tagging Technology
Xinxin Fang1, Jianping Chen1, Fei Yan2
1State Key Laboratory for Quality and Safety of Agro-products, Key Laboratory of Biotechnology in Plant Protection of MARA, Key Laboratory of Green Plant Protection of Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo, China.
Abstract:
Understanding the dynamic protein-protein interactions (PPIs) is fundamental to deciphering cellular signaling pathways and biological processes. Traditional methods for identifying PPIs, such as yeast two-hybrid and co-immunoprecipitation, often lack spatiotemporal resolution and may miss weak or transient interactions. Proximity-dependent biotinylation (PDB) coupled with mass spectrometry (MS) has emerged as a powerful technique to map the spatial proteome in living cells. Among various engineered biotin ligases, Turbidite-Identification (TurboID) stands out for its unprecedented catalytic efficiency, enabling rapid biotinylation of neighboring proteins within minutes. This chapter provides a detailed, step-by-step protocol for conducting a TurboID-based proximity labeling experiment to identify potential interacting proteins of a target protein of interest.
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