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Updated: May 15, 2026

Agroinfiltration and PVX Agroinfection in Potato and Nicotiana benthamiana
Published on: January 3, 2014
Phosphatidic Acid Application to Enhance Plant Virus Resistance
Jiayu Lin1, Wei Zhang2, Hongtao Zhang1
1The Engineering Research Center for Plant Health Protection Technology in Henan Province, College of Plant Protection, Henan Agricultural University, Zhengzhou, China.
None:
Phosphatidic acid (PA), a key signaling molecule in plant immunity, activates MAPK-mediated and reactive oxygen species (ROS)-mediated defense responses. Exogenous application of PA significantly reduces Potato virus Y (PVY) genomic RNA accumulation and alleviates disease symptoms in Nicotiana benthamiana, highlighting its potential as an immune elicitor for crop disease management. This chapter provides detailed protocols for PA solution preparation, MAPK pathway activation analysis (by Western blot and qPCR), and virus quantification to guide PA application for broader plant virus control in crops.
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