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Published on: February 3, 2020
Enhancing Plant Antiviral Resistance with Microbial Immunomodulators: A Half-Leaf Spot Screening Method
Chao Ma1, Kelei Han1, Dankan Yan1
1Institute of Plant Protection and Agro-Products Safety, Anhui Academy of Agricultural Sciences, Hefei, Anhui, China.
None:
The hypersensitive response (HR) represents a critical antiviral pathway in plants that can be leveraged for therapeutic screening via half-leaf spot assay. Attenuation of HR-induced necrotic lesions serves as a quantitative readout of antiviral efficacy. Here we present an integrated protocol combining: (1) rhizosphere bacteria isolation from asymptomatic plants in virus-infecting fields via serial dilution plating; and (2) quantitative antiviral assessment through necrotic lesion analysis. This comprehensive approach identifies microbial immunomodulators with potential for sustainable plant virus control.
