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Related Concept Videos

Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
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Updated: May 15, 2026

VIGS-Mediated Forward Genetics Screening for Identification of Genes Involved in Nonhost Resistance
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Published on: August 23, 2013

Development of a Reverse Genetics System for Rice Tiller Inhibition Virus.

Wencheng Liu1, Wenkai Yan2, Zhongxin Guo3

  • 1Shanghai Collaborative Innovation Center of Agri-Seeds/School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.

Methods in Molecular Biology (Clifton, N.J.)
|May 13, 2026
PubMed
Summary
This summary is machine-generated.

Developing a functional reverse genetics system for rice viruses is crucial for understanding and combating viral diseases that threaten global food security. This study details a method using rice tiller inhibition virus (RTIV) to enable molecular analysis and resistance breeding.

Keywords:
Infectious cDNA cloneRiceRice tiller inhibition virus

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Area of Science:

  • Plant pathology
  • Molecular biology
  • Agricultural science

Background:

  • Rice is a vital global food crop facing significant yield reduction due to viral infections.
  • Over a dozen rice viruses are known in China, but most lack functional reverse genetics systems, hindering research.
  • The absence of these systems limits molecular studies and the development of virus-resistant rice varieties.

Purpose of the Study:

  • To present a detailed protocol for establishing a viral reverse genetics system in rice.
  • To utilize the rice tiller inhibition virus (RTIV) as a model for developing this system.
  • To provide a framework for creating infectious clones of other rice viruses.

Main Methods:

  • Development of a viral reverse genetics system.
  • Utilizing rice tiller inhibition virus (RTIV) as a case study.
  • Establishing a protocol for creating infectious viral clones.

Main Results:

  • A detailed protocol for a viral reverse genetics system was successfully established using RTIV.
  • The developed methodology serves as a robust framework for future research.
  • This system facilitates molecular analysis and breeding for virus resistance in rice.

Conclusions:

  • The presented protocol is effective for establishing a functional reverse genetics system for rice viruses.
  • This framework supports the development of infectious clones for various rice viruses.
  • It is a significant step towards enhancing molecular understanding and breeding for rice virus resistance.