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Updated: May 15, 2026

Assessing Autophagic Flux by Measuring LC3, p62, and LAMP1 Co-localization Using Multispectral Imaging Flow Cytometry
Published on: July 21, 2017
Methods for Autophagy Detection by Fluorescence Microscopy
Xiaoye Ke1, Xianglong Zhang1, Xiangxiang Zhang1
1Pepper Germplasm Research Group, College of Agriculture, Anhui Science and Technology University, Chuzhou, Anhui, P. R. China.
Abstract:
Fluorescence microscopy is pivotal for investigating autophagy's role in plant antiviral immunity. Here, we present a standardized procedure using complementary probes, CFP-ATG8f for autophagosomal structures and monodansylcadaverine (MDC) for autophagic vacuoles, to assess autophagy during viral infection. This combined CFP-ATG8f and MDC staining system provides a powerful, reproducible method for evaluating autophagic activity in plant-virus interactions.
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