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Updated: May 15, 2026
A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes
Published on: March 25, 2014
High-Throughput Profiling of Antibody-Binding Epitopes Using AbMap
Wen-Jing Du1, Huan Qi1, Sheng-Ce Tao2
1Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University, Shanghai, China.
Antibodies play crucial roles in both basic research and clinical practice. The binding interface between an antibody and its antigen, i.e., the epitope, is a defining feature of the antibody. Accurate epitope mapping is essential for developing antibody-based reagents and therapies and safeguarding intellectual property. Here, we present the detailed procedure of a novel high-throughput technology, namely, Antibody binding epitope Mapping (AbMap) version 1.0. Leveraging versatile phage-displayed peptide library and next-generation sequencing (NGS), AbMap enables the comprehensive profiling of hundreds to thousands of antibody-binding epitopes in a single run.
Antibodies play crucial roles in both basic research and clinical practice. The binding interface between an antibody and its antigen, i.e., the epitope, is a defining feature of the antibody. Accurate epitope mapping is essential for developing antibody-based reagents and therapies and safeguarding intellectual property. Here, we present the detailed procedure of a novel high-throughput technology, namely, Antibody binding epitope Mapping (AbMap) version 1.0. Leveraging versatile phage-displayed peptide library and next-generation sequencing (NGS), AbMap enables the comprehensive profiling of hundreds to thousands of antibody-binding epitopes in a single run.
