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Updated: May 15, 2026

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes
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High-Throughput Profiling of Antibody-Binding Epitopes Using AbMap.

Wen-Jing Du1, Huan Qi1, Sheng-Ce Tao2

  • 1Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University, Shanghai, China.

Methods in Molecular Biology (Clifton, N.J.)
|May 13, 2026
PubMed
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We developed Antibody binding epitope Mapping (AbMap) 1.0, a high-throughput method using phage-displayed libraries and next-generation sequencing to profile antibody epitopes efficiently.

Area of Science:

  • Immunology and Biotechnology
  • Molecular Biology and Bioinformatics

Background:

  • Antibodies are vital tools in research and medicine.
  • Identifying the antibody-antigen binding site (epitope) is critical for antibody development and intellectual property protection.

Purpose of the Study:

  • To introduce Antibody binding epitope Mapping (AbMap) version 1.0, a novel high-throughput technology.
  • To provide a detailed procedure for comprehensive epitope profiling.

Main Methods:

  • Utilized a versatile phage-displayed peptide library.
  • Integrated next-generation sequencing (NGS) for data analysis.
  • Developed a high-throughput workflow for simultaneous epitope mapping.

Main Results:

Keywords:
AbMapEpitope mappingNext-generation sequencingPeptide screeningPhage display

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Last Updated: May 15, 2026

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  • AbMap 1.0 enables comprehensive profiling of antibody-binding epitopes.
  • The method allows for the analysis of hundreds to thousands of epitopes in a single experimental run.
  • Demonstrated a novel high-throughput approach for epitope discovery.

    • Conclusions:

    • AbMap 1.0 offers an efficient and scalable solution for antibody epitope mapping.
    • This technology advances antibody-based reagent and therapeutic development.
    • Facilitates accurate epitope characterization for research and commercial applications.