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Updated: May 15, 2026

Simultaneous Detection of Different Antibody Classes in a Multiplexed Serological Test
Published on: July 14, 2023
Simultaneous Affinity Maturation and Liability Removal from Antibodies
Camila Leal1, Fortunato Ferrara2, Andrew Raymon Morton Bradbury2
1New Mexico Consortium, Los Alamos, NM, USA.
Abstract:
Antibodies with affinities in the lower nanomolar or sub-nanomolar range or specific off-rate binding kinetic (Koff) are favored for many research, diagnostic, and therapy applications. Many protocols for affinity maturation exist but often fail to produce desired results due to inefficient exploration of the sequence space or because the developability of the parental molecule is compromised. Here we describe a two-phase protocol to explore the sequence space for a given antibody efficiently, enabling simultaneous affinity and developability maturation.
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