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Related Concept Videos

Affinity and Avidity01:41

Affinity and Avidity

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Antibody Actions01:26

Antibody Actions

Antibodies, or immunoglobulins, are critical players in the immune system's arsenal against invading pathogens. Produced by B cells and plasma cells, their primary role is to detect and bind to specific antigens, molecules found on the surface of pathogens like bacteria or viruses. Beyond antigen recognition, antibodies perform several vital functions that contribute to immune defense.
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Immunological memory, a pivotal pillar of the adaptive immune system, is responsible for the body's ability to remember and respond more swiftly and effectively to previously encountered pathogens. This remarkable feature is what makes vaccines so effective in preventing diseases.
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Special Features of Adaptive Immunity01:20

Special Features of Adaptive Immunity

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Related Experiment Video

Updated: May 15, 2026

Simultaneous Detection of Different Antibody Classes in a Multiplexed Serological Test
05:25

Simultaneous Detection of Different Antibody Classes in a Multiplexed Serological Test

Published on: July 14, 2023

Simultaneous Affinity Maturation and Liability Removal from Antibodies.

Camila Leal1, Fortunato Ferrara2, Andrew Raymon Morton Bradbury2

  • 1New Mexico Consortium, Los Alamos, NM, USA.

Methods in Molecular Biology (Clifton, N.J.)
|May 13, 2026
PubMed
Summary
This summary is machine-generated.

Developing high-affinity antibodies is crucial for research and therapeutics. This study presents a novel two-phase protocol for efficient antibody sequence exploration, simultaneously improving affinity and developability.

Keywords:
Affinity maturationDevelopabilityLiabilitiesLibrary designYeast display

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Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood
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Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood

Published on: February 6, 2018

Related Experiment Videos

Last Updated: May 15, 2026

Simultaneous Detection of Different Antibody Classes in a Multiplexed Serological Test
05:25

Simultaneous Detection of Different Antibody Classes in a Multiplexed Serological Test

Published on: July 14, 2023

Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood
13:14

Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood

Published on: February 6, 2018

Area of Science:

  • Biotechnology
  • Immunology
  • Protein Engineering

Background:

  • High-affinity antibodies (nanomolar or sub-nanomolar) are essential for research, diagnostics, and therapies.
  • Existing affinity maturation methods often struggle with inefficient sequence space exploration or compromising antibody developability.

Purpose of the Study:

  • To introduce a novel two-phase protocol for antibody engineering.
  • To achieve simultaneous affinity and developability maturation for antibodies.
  • To enable efficient exploration of antibody sequence space.

Main Methods:

  • A two-phase protocol designed for efficient antibody sequence space exploration.
  • Simultaneous optimization of antibody affinity and developability characteristics.

Main Results:

  • Demonstrated efficient exploration of antibody sequence space.
  • Achieved simultaneous improvements in antibody affinity and developability.

Conclusions:

  • The described two-phase protocol offers an effective strategy for antibody engineering.
  • This method facilitates the development of antibodies with enhanced affinity and improved developability for diverse applications.