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Fermentation-Driven Yeast Encapsulation as a Natural Strategy for Stabilizing and Delivering Mulberry Anthocyanins
Zexuan Li1, Xiang Yin1, Jiawen Wang1
1School of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou, Guangdong 510006, China.
A novel yeast, Hanseniaspora opuntiae DYG5, was identified to protect mulberry anthocyanins. A fermentation-adsorption strategy using this yeast enhances anthocyanin stability and delivery in functional foods.
Area of Science:
- Food Science
- Biotechnology
- Microbiology
Background:
- Mulberry anthocyanins offer health benefits but have poor stability and bioavailability.
- Existing methods for anthocyanin stabilization are limited, restricting their use in functional foods.
Purpose of the Study:
- To identify a yeast strain capable of protecting anthocyanins.
- To develop a fermentation-adsorption strategy for enhanced anthocyanin stability and delivery.
Main Methods:
- Identification and characterization of Hanseniaspora opuntiae DYG5 (DYG5) for anthocyanin adsorption.
- Development of a fermentation-adsorption (FA) strategy using inactivated DYG5 as a carrier.
- Structural analysis (SEM, TEM, BET/DFT, FTIR) of yeast cell walls.
- Assessment of anthocyanin stability under various conditions (light, heat, pH) and simulated digestion.
Main Results:
- DYG5 demonstrated faster adsorption kinetics and 25% higher capacity than Saccharomyces cerevisiae.
- Fermentation induced cell wall remodeling in DYG5, creating a distinct adsorption pattern.
- DYG5 FA significantly improved anthocyanin stability against light, heat, and extreme pH.
- Over 83% of anthocyanins were retained during simulated digestion, with sustained release.
Conclusions:
- Hanseniaspora opuntiae DYG5 is an effective anthocyanin-protective yeast.
- The fermentation-adsorption strategy provides a natural, food-grade method for enhancing anthocyanin stability.
- This approach improves anthocyanin delivery and release behavior for functional food applications.
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