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Published on: May 7, 2018
Comparison of 2D-LC and LC-MS/MS self- calibration internal standard method for the determination of quetiapine in
Mengli Ding1, Xiaodan Li1, Bi Zhang1
1Department of Clinical Laboratory, Qujing Third People's Hospital, Qujing, China.
Objective:
2D-LC method and LC-MS/MS self-calibrated internal standard method were used to compared the consistency and correlation of quetiapineserum concentration.
Methods:
Quality control samples at low, medium, and high concentrations of quetiapine, as well as serum samples from 138 psychiatric patients taking quetiapine, were analyzed using 2D-LC and LC-MS/MS self-calibrated internal standard method, respectively. Performed a complete validation of both methods. The Bland-Altman plot was used to assess the consistency of the results, while Pearson correlation analysis and Passing-Bablok regression were used to analyze the correlation among the results.
Outcomes:
The linear range for 2D-LC was 60.74-1518.48 ng/ml, and the linear range for LC-MS/MS self-calibrated internal standard method was 60.88-1522.02 ng/ml. For both methods, the relative standard deviation of intraday and daily precision was less than 15%. Calculated using the Bland-Altman plot, the 95.65% LoA was (-11.85, 30.82). The Passing-Bablok regression equation was OLZLC-MS/MS = 2.44 + 1.06 × OLZ2D-LC, and the Pearson correlation coefficient was 0.994 (p < 0.001).
Conclusion:
The 2D-LC and LC-MS/MS self-calibrated internal standard method, exhibit exceptional agreement and correlation in the quantification of quetiapine concentrations in serum. which can be used for conventional therapeutic drug monitoring (TDM).
