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Updated: May 15, 2026
Isolation of Precursor B-cell Subsets from Umbilical Cord Blood
Published on: April 16, 2013
Human leukocyte antigen study on a cohort of 149 Vietnamese umbilical cord blood samples
Kuo-Liang Yang1,2,3, Chi-Dung Phu4, Shee-Ping Chen1
1Laboratory of Immunogenetics, Tzu Chi Cord Blood Bank, and Buddhist Tzu Chi Bone Marrow Donor Registry, Buddhist Tzu Chi Stem Cells Centre, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien, Taiwan.
Objectives:
We here report the distribution of the human leukocyte antigen (HLA)-A, -B, -C, -DRB1, and -DQB1 alleles among 149 units of Vietnamese umbilical cord blood samples and deduced their most probable HLA haplotypes for HLA-A/B loci, HLA-B/C loci, HLA-DRB1/DQB1 loci, HLA-A/C/B loci, HLA-A/C/B/DRB1 loci, and HLA-A/C/B/DRB1/DQB1 loci. Our objective is to reveal the unprecedented information on the distribution of HLA alleles in the Vietnamese cord blood and to find out the most probable HLA haplotypes associated with the various HLA alleles detected in the cohort of 149 cord blood specimens.
Materials And Methods:
The material for this study was umbilical cord blood samples, preserved in acid citrate dextrose anticoagulants. The blood donors were umbilical cord blood donors whose parents voluntarily donated their newborn baby's cord blood to the Blood Transfusion and Hematology Hospital, Ho Chi Minh City, Vietnam. DNA sequence-based typing of the Sanger's sequencing method was performed for the HLA allelic typing. Target exon sequence amplifications were achieved by polymerase chain reaction (PCR), and the resulting amplicons were sequenced by BigDye Terminator Cycle Sequencing Ready Reaction Kit, according to the manufacturer's protocols.
Results:
In the total number of 149 randomized cord blood samples studied, we detected distinctive 22 HLA-A alleles, 23 HLA-C alleles, 44 HLA-B alleles, 27 HLA-DRB1 alleles, and 15 HLA-DQB1 alleles. In addition, we determined the most probable HLA haplotypes for the alleles detected.
Conclusion:
Our findings in this study are useful in research for the comprehensive understanding of the distribution of HLA alleles and their associated HLA haplotypes in Vietnamese cord blood. Further, the cord blood with HLA typing determined is now ready for matching search purpose for patients who are looking for suitable hematopoietic stem cell donations for transplantation therapeutic applications.
