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Related Experiment Video

Updated: May 16, 2026

A Protocol to Evaluate and Quantify Retinal Pigmented Epithelium Pathologies in Mouse Models of Age-Related Macular Degeneration
09:24

A Protocol to Evaluate and Quantify Retinal Pigmented Epithelium Pathologies in Mouse Models of Age-Related Macular Degeneration

Published on: March 10, 2023

RPE With ROCK-Mediated Epithelial-Mesenchymal Transition as a Key Contributor of Subretinal Fibrosis in AMD.

Iori Wada1, Keijiro Ishikawa1, Muneo Yamaguchi1

  • 1Department of Ophthalmology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.

Investigative Ophthalmology & Visual Science
|May 14, 2026
PubMed

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Summary

Subretinal fibrosis in neovascular AMD originates from RPE cells via ROCK2-mediated EMT. ROCK inhibitors like Ripasudil show promise for treating this condition.

Area of Science:

  • Ophthalmology
  • Cell Biology
  • Molecular Medicine

Background:

  • Subretinal fibrosis (SRF) is a major cause of vision loss in neovascular age-related macular degeneration (nAMD).
  • The cellular origins and molecular drivers of SRF remain incompletely understood.

Purpose of the Study:

  • To investigate the cellular origin of SRF in nAMD.
  • To elucidate the molecular mechanisms of SRF, focusing on RPE cells and Rho-associated coiled-coil-containing protein kinase (ROCK)-mediated epithelial-mesenchymal transition (EMT).

Main Methods:

  • Utilized fate-mapping with lineage tracing to identify cell sources.
  • Employed microarray, RT-PCR, and immunohistochemistry for molecular analysis.
  • Assessed the role of ROCK pathway and EMT using ROCK inhibitors (Ripasudil, Belumosudil) in functional assays.

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LipidUNet-Machine Learning-Based Method of Characterization and Quantification of Lipid Deposits Using iPSC-Derived Retinal Pigment Epithelium
06:16

LipidUNet-Machine Learning-Based Method of Characterization and Quantification of Lipid Deposits Using iPSC-Derived Retinal Pigment Epithelium

Published on: July 28, 2023

Related Experiment Videos

Last Updated: May 16, 2026

A Protocol to Evaluate and Quantify Retinal Pigmented Epithelium Pathologies in Mouse Models of Age-Related Macular Degeneration
09:24

A Protocol to Evaluate and Quantify Retinal Pigmented Epithelium Pathologies in Mouse Models of Age-Related Macular Degeneration

Published on: March 10, 2023

LipidUNet-Machine Learning-Based Method of Characterization and Quantification of Lipid Deposits Using iPSC-Derived Retinal Pigment Epithelium
06:16

LipidUNet-Machine Learning-Based Method of Characterization and Quantification of Lipid Deposits Using iPSC-Derived Retinal Pigment Epithelium

Published on: July 28, 2023

Main Results:

  • Identified retinal pigment epithelium (RPE) and endothelial cells as myofibroblast sources.
  • Observed upregulation of EMT markers and ROCK1/2 expression in SRF lesions.
  • Demonstrated that RPE-specific ROCK2 knockout and Ripasudil treatment significantly reduced SRF and EMT.

Conclusions:

  • RPE-derived myofibroblasts, through ROCK2-mediated EMT, are key drivers of SRF in nAMD.
  • ROCK inhibitors, such as Ripasudil, represent potential therapeutic strategies for nAMD-associated fibrosis.