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Updated: May 16, 2026
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Monitoring Protein Adsorption with Solid-state Nanopores
Published on: December 2, 2011
Development of a 16-channel solid-state nanopore array platform for integrated nanopore fabrication and ionic current
Itaru Yanagi1, Tadashi Kiyuna2, Keiko Esashika3
1Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo, 113-0033, Japan. i-yanagi@g.ecc.u-tokyo.ac.jp.
Lab on a Chip
|May 14, 2026
Summary
A new 16-channel solid-state nanopore system enables parallel fabrication and measurement. This advanced system improves throughput and molecular discrimination for DNA analysis using dielectric breakdown fabrication methods.
Area of Science:
- Nanotechnology
- Biophysics
- Materials Science
Background:
- Parallel solid-state nanopore measurements promise enhanced throughput and molecular discrimination.
- Existing methods require further development for efficient parallel fabrication and precise control.
Purpose of the Study:
- To develop and demonstrate a 16-channel solid-state nanopore array measurement system for parallel fabrication and ionic current recording.
- To evaluate nanopore fabrication modes and their impact on molecular translocation detection.
Main Methods:
- Development of a 16-channel solid-state nanopore array system.
- Utilized two fabrication modes: pulsed-voltage-induced dielectric breakdown (MPVI) and controlled dielectric breakdown (CBD).
- Conducted experiments with single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) translocation detection.
Main Results:
- Fabricated nanopores with diameters from 1-2 nm (MPVI) and 8-10 nm (CBD) with tight size control across most channels.
- Evaluated pore-diameter dependence of dwell time and current blockade amplitude for ssDNA.
- Observed dsDNA translocation events in all 16 channels using CBD-fabricated nanopores.
Conclusions:
- The developed 16-channel system enables parallel nanopore fabrication and measurement, significantly advancing throughput and molecular analysis capabilities.
- The MPVI and CBD fabrication modes offer versatility in creating nanopores for diverse molecular detection applications.
- The system demonstrates high performance and precise control, paving the way for advanced nanopore sensing applications.
