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Updated: May 16, 2026
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Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications
Published on: June 23, 2016
PolyA-tail-mediated assembly of a gold nanoparticle-based turn-on fluorescent aptasensor for ultrasensitive detection
Zhuo-Ying Li1,2, Li-Xia Yan3, Xu Zhao1,2
1Institute of Analytical Food Safety, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
Abstract:
A turn-on fluorescent aptasensor for PFOA is developed using a polyA-tailed aptamer anchored on gold nanoparticles and a fluorescently labeled cDNA. The displacement of cDNA by PFOA restores fluorescence, enabling ultrasensitive detection with a 1.3 nmol L-1 limit, demonstrated by satisfactory recovery in spiked fish samples.
